Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

What test should I use to compare two or three means, if data is not normally distributed but sample size is > 30 in each group?

I personally feel this as a most confusing question. As per my understanding, if sample size is > 30, and sample is skewed, we can still use paramteric approach, as it will follow Z...

01 March 2021 1,587 6 View

Why am i not getting solid after reacting APTES with salicylaldehyde?

The reaction of APTES with salicylaldehyde in ethanol leads to the formation of imine compound yellow solid which is mention in literature, but I am not getting yellow solid I don't know why? when...

28 February 2021 2,955 2 View

How to calculate Ligand-active site distance in a ligand-protein complex from MD simulation?

I have run a MD simulation of a ligand-protein complex in GROMACS using CHARMM forcefield. Reviewer has asked me to calculate Ligand-activesite distance and binding free energy of ligand with...

27 February 2021 4,703 1 View

Efficiency of Proximity Feed Patch Antenna ?

Greetings to all of You! I have searched some research papers for the designing of a proximity coupled patch antenna. Researchers are talking about the impedance bandwidth of the antenna but...

24 February 2021 7,662 3 View

Please help in identification of this insect?

This insect is minute in size around 1 mm and not clearly visible with naked eye.

22 February 2021 5,907 11 View

What is causing the raw fluorescence to be negative?

Hi everyone, I would really appreciate it if someone can tell me when and why a raw fluorescence starts below zero. In the picture attached below the blue colour curve starts from a negative...

09 February 2021 4,642 3 View

How to match the XRD of a compound for which there is no JCPDS file ? Is there any other characterization to claim the synthesis of desired compound?

I have synthesized LiMgF3 using the starting precursors LiF and MgF2 via solid state diffusion method. Now I am looking for some characterization to claim the desired product, as there is no...

09 February 2021 921 3 View

Can anyone suggest me broad host range fluorescence expression vectors for Gram-positive bacteria ?

Hi everyone, I am looking for fluorescence expression vectors for expression in gram-positive bacteria present in the skin microbiome. I need to tag them with a fluorescence marker and observe...

06 February 2021 2,566 4 View

Are the hyperspectral image datasets which are publicly available at ehu.eus website atmospherically and geometrically correct?

I've come across a couple of publicly available hyperspectral image datasets from the website http://www.ehu.eus/ccwintco/index.php/Hyperspectral_Remote_Sensing_Scenes Now, my concern is...

05 February 2021 3,550 1 View

SSR primers are species specific or we can use it for any plant species ?

Hi, any one please guide me; SSR primers are species specific or trait specific. i have few primer which was reported in articles for wheat yield can i use these similar primer for lentil yield...

20 February 2021 315 4 View

Why this SSR primer show band like that ?

Each time this SSR primer show band like that other show one or two band . Is it polymorphic ?

11 January 2021 2,221 5 View

What is the best quality DNA for SSR fingerprinting?

Nanodrop result of my DNA samples extracted from fresh finger millet leaves showed 260/280 ratio ranged from 1.7-2.2 whereas 260/230 ratio ranged from 1.1-2.2. How can I assess the quality of such...

30 November 2020 8,689 6 View

Weak SSR peak on capillary electrophoresis trace - is it a heterozygote?

Looking at the attached image, the top two samples show a weak peak at 212 compared to the very strong 200 peak. The bottom samples is homozygous for the 212 peak. Since the samples look a bit...

27 October 2020 2,353 3 View

What tool can I use for creating a Quantative Trait Loci map from SSR data?

I have SSR data for some individual of three hybride How I construct QTL mapping of yield according to these data ?

02 October 2020 502 5 View

How to calculate marker-trait association?

there are many method to calculate marker-trait association, namely stepwise regression, GLM, etc. Before running those methods, how we define the X (independent variables) and Y (respon...

14 September 2020 6,689 2 View

No amplification of SSR markers on Pecan nuts project?

Hi all, I am doing an SSR project on Pecan nuts and I am struggling to get amplification on my PCR. Here is a summary of what I have done so far 1. DNA extraction was a modified CTAB protocol...

19 April 2020 8,678 2 View

Running multi-locus SSR binary data on GenAlEx?

I have data from SSR markers that have 2-10 bands each. I was able to run analysis wherein the program consider each band as single loci. The program gave me the following data (as in attached...

24 February 2020 6,318 1 View

Which software is good for analysis of band size in gel image? How can we analyze the curve band image?

Currently, i am thinking about GelAnalyzer software. Please help me with the analysis and band scoring. I have used 100 bp ladder.

17 February 2020 7,762 5 View

Which software is good for analysis of band size in gel image? How can we analyze the curve band image?

Currently, i am thinking about GelAnalyzer software. Please help me with the analysis and band scoring. I have used 100 bp ladder. PIC 1 and 2 are different image.

01 February 2020 4,566 4 View