What is the role of ether solvent (such as THF) in Sonogashira coupling?

Can Amine (such as Et3N) be used as solvent as well as base for the Sonogashira Coupling? How to decide the amount of solvent to be used in any reaction? Does the amount of solvent affect the...

14 February 2021 4,018 3 View

How Architecture is related with Culture?

How architectural form, shape, style and technique are influenced by Cultural Values and Norms?

05 October 2020 6,651 17 View

Do we really pay serious attention to balance such factors for good design affecting in Human comfort?

It has been recognized that Architecture is influenced by many factors like, Social, Physical, Cultural, Environmental, Economic and Political etc.

04 October 2020 5,867 10 View

Which circuit design should I use to generate emf oscillating at frequency of 2.5Ghz ?

I am looking for a circuit which can generate an emf oscillating at frequency of 2.5Ghz. My setup is in such a way that, I am passing particles (negligible mass) from a tunnel and want an emf...

21 December 2019 9,800 6 View

Why Biosurfactant production varies with the carbon sources?

Some Pseudomonas sp. are reported for biosurfactant production with glucose as a carbon source, where as other Pseudomonas sp. reports the significant BS production with hydrocarbon. Why?

12 November 2019 282 3 View

What is the role of nitrobenzene in the synthesis of 1,3,6,8-tetrabromopyrene ?

During the synthesis of 1,3,6,8-tetrabromopyrene, nitrobenzene was used as solvent, so I feel that it was used as a solvent may be because the reaction requires high temperature to form the...

03 July 2019 2,534 3 View

Researching a plant which is known to be anticancerous, as it is referred in ethno-medicine,its molecule is cited should wet lab be done to verified ?

The plant is already known as anti-cancerous, but the biochemical study has not been done. However the active compound, from the plant has been researched already to find anti-cancerous activity...

13 June 2019 451 5 View

What can possibly replace Aluminium in Aerospace industry?

I am looking for material which can replace aluminium in aerospace industry as i want material which will be having high melting point and must be light in weight. As i am looking at this from...

20 May 2019 9,612 28 View

How to take PL of Methylammonium Lead iodide thin film?

I am working on methylammonium lead iodide perovskite thin films. Whenever I try to take PL of MAPbI3 thin films using Agilent Cary Eclipse Fluorescence Spectrophotometer I didn't get any peak....

11 April 2019 5,176 4 View

Is there any library in python for evaluationary algorithm?

evolutionary algorithm

02 April 2019 8,065 16 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Antibody anti-IgM Bovin conjugated Biotin ?

Hi everybody, Can anyone please suggest me an antibody anti-IgM Bovin conjugated Biotin ? I would like to use it in LUMINEX with a streptavidin conjugated phycoerythrin reporter. Thanks in...

25 February 2021 9,659 2 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View