Hi  Aniruddh 

You can do manually the grain size measurement by a well known, old fashion, method as the following steps:

1-Draw five lines across the image.

2-For each line, count the intersection of the line with each grain boundary , of the present grain,that the line encounters, including the last intersection of the line with the edge of the image, but not the first edge of the image.

3-Divide the length of the line by the number of intersection that you were encountered.

This gives you the grain size of one line.

4-Repeat the above with the rest of the four lines.   Eventually, you will have 5 recordings of the grain size, then take the average value of the five recordings & try to obtain the standard of deviation, hoping that the standard of deviation falls within a reasonable range, +/-0.0-5%, with an error bar  of 10%, that  would be fine.  

Hi Aniruddh,

Thanks for posting. I have used our new and rapidly growing image analysis software MIPAR (http://MIPAR.us) to automatically find and measure your grains with a single Recipe. I have attached the Recipe file as well as the grain segmentation it produces. Just open the image in MIPAR's Image Processor, load the recipe, and you're done!

The recipe will then also measure the total area of all grains (A), as well as their count (N), such that an estimate of grain size can be A/N. It also runs a mean linear intercept measure of grains, and reports the ASTM grain size number.

Please let me know of any questions - we are always here to help. Everyday we are so warmed users (of  ImageJ and others) who could not solve their problems with classical software, or were very frustrated, and are thrilled to find MIPAR.

Cheers,

John

Dear John

Keep up the good work.

Khaled

Dear Khaled,

Thanks very much indeed - means a lot.

Cheers,

John

Dear Anirudh,

You can use WsxM image developer software. I hope you will enjoy that because everything is present there including grain size measurement!

Hi Aniruddh,

In my case am using Gwyddion, its free and allows fast and simple image analysis.

you have just to adjust a threshold and then the software gives you all the informations concerning the diameter, the height, the count of your grains, etc...

here u find the analysis of your image, by this software, showing the mean diameter of each grain (btw, i dont knwo the z height of your image, so i used a relative one)

Best regards

Answers are interesting. I will give  some software  suggested here a trial

Hi Aniruddh,

Did you use the Gwyddion to estimate the particle size?

Hi

Refer ro the link:

(https://www.google.com.kw/search?rlz=1C1CHZL_enKW766KW766&biw=911&bih=438&tbm=isch&sa=1&ei=3FoCXNGWHoGnsgG_7IHgAg&q=+software+for+image+analysis&oq=+software+for+image+analysis&gs_l=img.3..0i24.15058.15058..16286...0.0..0.480.480.4-1......0....1..gws-wiz-img.i3ysWY1xZrE)

It can be done by gwyddion

Gwyddion is simply to used

Can anyone tell me the steps to calculate the grain size using Gwyddion?