Alternative method of Permeablization in IL17A assay by flowcytometry?

I am evaluating Th17 cells by staining stimulated cells with IL17A and IFN-Y antibody by flow cytometry. Permeabilization step was done with Cytoperm/cytofix reagents (BD Bisciences) yet and the...

01 December 2020 4,553 1 View

Suggestions how to get Shapefile of India with all 700+ Indian districts?

I need shapefile for all 700+ districts of India. From where I can find the India shapefile with all districts. Also, suggest how to get the latest India shapefile with all state and union...

07 August 2020 3,449 5 View

PSO, ABC or GA, which one to choose while solving Mixed Integer Programming problem?

I am interested to solve a mathematical problem (MILP) using evolutionary algorithms but confuse about which one to choose as a beginner in the programming languages. Suggest an algorithm easy to...

13 July 2020 4,027 51 View

What is the best tool for adding references (in a research paper) within a word file?

What is the best tool for adding references (in a research paper) within a word file

16 June 2020 8,275 9 View

How can I check the viability of RAPD Primers?

Dear Researchers Good day to you I want to ask if we can check our RAPD primers ( both in diluted & lyophilized forms) on agarose gel electrophoresis whether they are degraded or not as our...

24 February 2020 9,172 3 View

How can I develop an ANFIS model for WQI in matlab?

I want to implement the Water quality index in ANFIS. I have used constant output, but not getting the desired output.

06 February 2020 3,394 4 View

Can food supply chains be secured by blockchain diffusion???

Blockchain technology promises to provide complete information security and elimination of intermediary alteration. But can we really full utilize this feature to food supply chain. How can we...

03 May 2019 5,476 2 View

When the unit of analysis is 'supply chain' or 'organization', is it acceptable to take more than one respondent from an organization?

Question related to research methodology

06 March 2019 9,150 7 View

No title

02 May 2018 368 2 View

How to generate UDF in Fluent to measure Droplet concentration at a particular downstream in Turbulent flow?

I don't know how to write UDF. If you know then Please help me..or you can also write the UDE for me and Send me.

22 February 2018 1,883 3 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

How to code open-ended questions in SPSS?

Hello all, In SPSS I am going to code 2 open-ended questions. I have already read all the answers and I made a list of the most important categories to which I can code the answers. This question...

02 March 2021 1,757 4 View

How can I integrate a routing protocol in a tiny device (e.g. Raspberry Pi)?

Hi Community, I'm facing the issue of integration/compiling a new routing protocol in a WSN simulator. The final goal is to successfully add, configure this routing protocol in hardware devices...

01 March 2021 9,332 6 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

How can we simulate an hyperelastic-viscoelastic behavor with Ansys APDL?

I am working on modeling and simulation of biomecanical material behaviour, I have succeded on simulating skin using anisotropic hyperelastic material, on APDL and in our lab's finite element...

28 February 2021 552 3 View

Modeling viscosity using model of Chung?

Hello, As part of simulation of gases mixture and water, I need to calculate viscosity of the fluid components and I am using relationships proposed by Chung et al. (1988). The irony is while I...

25 February 2021 8,053 5 View

Satellite orbit estimation using Recurrent Neural Network - what should I use?

Hello, I am a master's student studying in Yonsei Univeristy, Korea. I am trying to estimate the state of satellite, using Neural Network. Below is a simple flow of my study. 1. Train (t0 ~ t1)...

23 February 2021 6,383 3 View

Fictitious Boundary Meshing Method (FBM) in Commercial FEA software?

Is it possible to implement FBM in a commercial FEA software package, such as FEMLAB, COMSOL, or open source software like Moose?

22 February 2021 2,968 1 View

How to find out the problem of VMD software when i run it?

Hello I try to install and run the VMD software on windows 10 (64-bit).It doesn`t work and it is closed when I run it. I checked all of tutorials, videos and documents. But they did not work...

22 February 2021 8,211 3 View

How can I get the coding gene sequences of Hela and U2OS cell lines? Is mRNA sequence of Hsp90 alpha tissue specific??

I am looking for hsp90 mRNA sequence in Hela and U2OS cell lines, I have tried through ncbi but always the output is hsp90 in homo sapiens not in the specific cell line. Kindly, could any one...

22 February 2021 1,853 3 View