How to clone fragments into vector without adding extra space?

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Alexandra Johnson

Gibson Assembly or any of it's derivatives like NEBuilder. Explanation video: https://youtu.be/LwOYvWTm7kE
Overlap extension PCR. Explanation video: https://youtu.be/g-eaYS3k3JI
Golden Gate cloning. Explanation video: https://youtu.be/piyc2ONyV1o

Any of these methods will let you stitch together multiple fragments without restriction recognition site "scars" within the coding sequence.

Suipwksiow Wjkp

@Alexandra I just watched all three videos. Very helpful! Thanks a lot!

Glad I could help! Hope your cloning goes well!