09 September 2017 3 2K Report

I'd like to fuse 3 fragments into a vector, but they have RE sites. How to connect them together without adding extra space? I don't wanna additional a.a to be translated.

More Suipwksiow Wjkp's questions See All
How to ensure a marker antibody won't activate T cells and affect the result?

I'm recently buying some anti-CD3 and anti-CD28 to activate T cells. I also notice some of this clones have been conjugated with fluorescence for the purpose of labeling T cells for flow...

09 October 2019 1,095 4 View

Why my gene in single-cell sequencing showed zero value?

I have many single-cell sequencing data. I checked my gene of interest in these data. However, all of these single-cell sequencing data showed that my gene has no expression, no matter in immune...

31 December 2018 3,817 3 View

Why can't I detect the surface protein by western blot?

I was doing an over-expression experiment of a gene for a year. It is a surface protein. The biggest trouble is that I can never detect the gene overexpression by western blot. Interestingly, when...

10 November 2018 7,335 3 View

Why some cancers are not prone to mutate?

When I first learn cancer biology, my teacher told me that cancer is usually caused by the loss of tumor suppressor , which is like you loss your brake. After it losses the suppressor, it tends...

05 June 2018 8,193 2 View

Are there any cancer cell lines that can form tumor in nude mice but not in normal (immune competent) mice?

Are there any mouse cancer cell lines that can form tumor in nude mice but not in normal (immune competent) mice? Please give me some examples. Thanks.

04 May 2018 6,810 2 View

What are the current applications of Artificial Intelligence in biology or cancer?

AI seems to be pretty popular these days and there are many applications in our daily life. But are there any AI designed to help our researchers to solve any biology questions?

04 May 2018 4,158 3 View

Why don't use ampicillin to select transduced mammalian cells?

Why don't use ampicillin to select transduced mammalian cells? also, I saw some lenti-vector is Bleo Resistant, is bleo used as bacteria selector or mammalian cells selector?

03 April 2018 7,783 0 View

Can I cut the two ends of a gene with same restriction site and clone it to a vector?

The two ends of my gene have same restriction sites. And the destination vector also has two same sites. Can I clone the gene directly to the destination vector? I know it's possible that the gene...

03 April 2018 8,449 4 View

What's the antibiotic cell selection for plasmid FUGW?

According to the website https://www.addgene.org/14883/, it has bleomycin resistance. However, bleomycin can be used both for bacteria selection and cell selection. Then what is the cell...

03 April 2018 3,923 1 View

Can I use NEB mutagenesis- substitution method to clone sgRNA into the vector?

Can I use NEB mutagenesis -- substitution method to clone sgRNA into the vector? I felt it's much easier. Just design the primers containing the sgRNA bp, conduct PCR and do a ligation. And most...

02 March 2018 684 1 View

Similar questions and discussions
Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

Is there an English Translation of the Carl Moller text: ZUR VERGLEICHENDEN ANATOMIE DER SILURIDEN?

I recently came across an anatomy text by Carl Moller that was published in 1915 but it is in German or Dutch neither of which I can understand. I would like to know if there is an English...

10 August 2024 4,347 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

How can major oxides determined by ICP-MS/ES be normalized to 100% volatile free? How does this connect with LOI?

I am analyzing cherts from different sources found within one specific geologic formation using ICP-MS/ES. My committee was asking this question about my analysis. I was not sure how to answer this

05 August 2024 7,902 3 View

Is it true that the vacuum bomb is about to arrive?

We assume this to be true. We also assume that the vacuum bomb is the latest version of explosives with an explosive power of a few to ten kg of TNT equivalent. It has the unique characteristic of...

04 August 2024 4,534 1 View

How to load and plot 2D-PIV (particle image velocimetry) recorded velocity vector field in Techplot?

Dear Researchers I need to know, how to load and plot 2D-PIV (particle image velocimetry) recorded velocity vector field data in Tecplot? Thank You

02 August 2024 3,615 1 View

How can we now if the promotor in the vector is well?

Hi, everyone. I want to transfer two genes in to the pseudomonas bacteria that isolated from the soil. The bacteria that I want to use for cloning haven't been identified and not be sequenced,...

02 August 2024 3,987 1 View

How do environmental toxins interact with genetic predispositions to increase cancer risk?

I am currently researching the impact of environmental toxins on children's health and would greatly appreciate insights from experts in the field. If you are an expert or researcher working on...

02 August 2024 2,627 1 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

Illustra™ MicroSpin™ G-25 columns what it is used for?

Hello, We found three packages of Illustra™ MicroSpin™ G-25 columns in the cabinet of an unused lab. They are very old but have never been opened. I have never used this kit before, and I couldn't...

25 July 2024 4,927 3 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close