How to clone fragments into vector without adding extra space?

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Why some cancers are not prone to mutate?

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Are there any cancer cell lines that can form tumor in nude mice but not in normal (immune competent) mice?

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What are the current applications of Artificial Intelligence in biology or cancer?

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Why don't use ampicillin to select transduced mammalian cells?

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Can I cut the two ends of a gene with same restriction site and clone it to a vector?

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Expression cloning by using cDNA,do I need to modify the cDNA first?

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Alexandra Johnson

Gibson Assembly or any of it's derivatives like NEBuilder. Explanation video: https://youtu.be/LwOYvWTm7kE
Overlap extension PCR. Explanation video: https://youtu.be/g-eaYS3k3JI
Golden Gate cloning. Explanation video: https://youtu.be/piyc2ONyV1o

Any of these methods will let you stitch together multiple fragments without restriction recognition site "scars" within the coding sequence.