I am quantifying plant metabolites using HPLC, The solvents in channel A is 0.4% phosphoric acid in water and in channel B it is acetonitrile. after performing the experiment which solvents I should use to wash the column.
Answers will be highly appreciated
Thank you
Wash the column with 70% water; 15% methanol; 15% acetonitrile. Divert the column eluent to waste not to contaminate your detector(s). Wash the column slowly over to 100% methanol and wash for at least 15 minutes. Wash the column over to 100% acetonitrile and wash for at least 15 minutes. Depending on the type of column you are using you can store the column in 100% organic depending on the manufacturer's recommendations.
Regards
Bachir Achour Thank you for your answer. For further clarification, I have been asking after washing with 70% water, 15% MeOH and 15 acetonitrile. Then I have to wash the column with 100% MeOH for 15 mins and then 15 mins with acetonitrile?
Dear Muhammad,
Yes, absolutely. After washing with 70% water, 15% MeOH and 15 acetonitrile, then wash the column slowly over to 100% methanol and wash for at least 15 minutes. Wash the column over to 100% acetonitrile and wash for at least 15 minutes.
Regards
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