We are currently using the chemical A-804598 (see attached file for molecular structure; molecular weight 315.37) in a mice in vivo study. The substance is soluble in dimethylsulfoxide (DMSO) to 100nM, but some literature suggested that DMSO might have neuroprotective effect (which is our research interest) and toxicity built up overtime. As a 56 day in vivo treatment, what alternative solvent can we use, that has a lower toxicity/side effect? Much appreciated!
A hydroxy-cyclodextrin may enhance the delivery of your compound and is not toxic or neuroprotective when dissolved in water.
Many metabolically important compounds, such as lipid-soluble vitamins and hormones, have very low solubilities in aqueous solutions. Various techniques have been used to solubilize these compounds in tissue culture, cell culture, or other water-based applications. A frequently used approach is to use cyclodextrin as a “carrier” molecule to facilitate the dissolution of these compounds.
DMSO has an antioxidant effect that makes it neuroprotective: http://www.ncbi.nlm.nih.gov/pubmed/21059397. It is quite a long in-vivo study you are planning so I think you should avoid or reduce DMSO as much as possible. Not more than 5% - you can then have a second co-solvent like PEG400. Or try a nanosuspension or other non-conventional formulation.
- Badges
- Science topic