This is a slight follow-up to a question I asked a month ago, but I had isolated sperm from my mice and froze pellets and kept tissue samples at -80C. There was a miscalculation on my end for one of the components of buffer used to extract sperm (pyruvic acid was 14X higher than it needed to be). I eventually want to investigate small non-coding RNA populations in these sperm samples; however, I want to ensure that the samples have not been compromised by my buffer mishap. I've been actively searching for protocols/methods to assess RNA or sncRNA populations in my tissue samples but to no avail. Does anyone have any insights into possible quality control methods I can perform on my sperm samples to assess if they're viable for future analyses? Thank you!!