How to check anticancer activity of rice seeds?

More Lahiru Welikanna's questions See All

How to find, 5' UTR and 3' UTR regions in a given mRNA strand?

Dear colleagues, When we have a given mRNA sequence, how can I find the exact sequences of its promoter, 3'UTR and 5'UTR regions which lie inside it? Are there any specific analyzing tools? If...

07 July 2021 7,161 2 View

What is the best way to optimize the crosslinker concentration for protein interaction analysis?

Dear All, What would be the best strategy to optimize a certain crosslinker concentration for my protein interaction studies? Thanks

06 May 2021 1,658 4 View

Why is it difficult to dissolve disodium EDTA to prepare a 0.5M EDTA (pH 8.0) solution?

Dear scientists/ colleagues, I am curious to prepare a 500mL of 50× TAE buffer (Tris Acetate EDTA): using 121g of Tris base(121.14 g mol-1) + 28.55 mL of glacial acetic acid + 50 mL of 0.5 M...

19 March 2021 9,964 2 View

Are there any alternative sources for sugar except isomalt( Glucose) and maltitol(Sucrose) ?

Are those available in Sri Lanka and what about its market price?

07 March 2021 1,555 2 View

How can we provide a guarantee of privacy for a GPS based contact tracing system to ensure public engagement to gather data?

With the current pandemic situation around the world, it comes to attention that if contact between people is tracked (via mobile devices - GPS), people could take much safer precautions by...

16 April 2020 2,989 6 View

How to find/predict transcription factors for a putative promoter of the gene of my interest?

Dear All, Based on ORCA conserved regions, CpG islands, and histone methylation, I predict a putative promotor for my gene of interest with one of my colleague's help. I need to find/predict...

21 February 2020 3,253 3 View

What are the best methods to estimate ammonia volatilization by different nitrogen fertilizers?

Currently, I m developing a new fertilizer from organic and inorganic substances. I need to quantify how much nitrogen loss by ammonia volatilization in actual field level. Can anyone recommend...

04 February 2020 3,510 3 View

What is the best method to determine, plant available Si in Sugarcane Growing soils (is it 0.01 M CaCl2 method) ?

I have been working on a research regarding Si fertilizer application on Sugarcane crop. Many literature indicates that 0.01M CaCl2 method is most suitable. If it so can anyone provide the correct...

28 August 2019 9,607 12 View

If I use a 5 point Likert scale to measure DV , what is the most apporiate Regression to run ?

My IV's are both Scale and Nominal.

19 June 2019 9,771 0 View

Can any one explain a method to estimate the nutrients releasing pattern of a particular fertilizer ( specially organic) ?

I am working on a project to produce an organo-mineral fertilizer. I need a scientific method to determine nutrients releasing pattern of that new fertilizer. Can anyone suggest a scientific method?

26 March 2019 295 1 View

What is the most effective way to sterilize camelina seeds?

I'm working on camelina seed sterilization and have some doubts about the process. I followed a protocol used by my lab mate, but I'm unsure about a couple of things: Is it safe to use 10% bleach...

02 August 2024 1,178 3 View

When should I stop watering A.thaliana for collect their seeds?

I have been following Rivero et al 2014 protocols, but I am not sure when I should stop watering because my plants are completely yellow but the siliques are not 80% brown. Any recommendations?...

30 July 2024 2,301 7 View

How should I prevent mould contamination on small seeds in a petri dish exposed to warm temperatures?

Hello all, Recently as part of a research project at university, we have been studying the effects of light and temperature on eucalyptus seed germination. In our initial run of trials we...

24 July 2024 8,850 4 View

HEC 1A & HEC1B Cell Lines?

Hi, Kindly guide me that how many cells of HEC1A & HEC1B Cell lines should I seed for Wound healing assay and which plate type is recommended 6, 12 & 24?. Articles suggested mainly 24...

20 July 2024 4,143 2 View

Iwork on producing organic fertilizer from rice husk. how can I reduce the bioconversion time ?less than two months?

I work on producing organic fertilizer from rice husk. how can I reduce the bio-conversion time ?less than two months

20 July 2024 6,049 3 View

How to improve my plaque assay for influenza virus?

I used MDCK cells for my plaque assay for influenza virus and seeded 950,000cells/well in 6-well two days before infection. The virus was diluted in FBS-free MEM with 0.5ug/ml TPCK-trypsin. Here...

11 July 2024 5,669 5 View

How to convert protein percentage into kg/ha?

I have protein percentage and Seed yield per hectare data, how to calculate protein content kg per hectare.

11 July 2024 9,045 4 View

How can I determine the percentage of cell viability of cells seeded in 96 well plate ?

I am currently testing the effect of bacterial filtrates on cancer cells , after seeding the cells I tested the bacterial filtrates against them , and got images of all 96 wells using inverted...

10 July 2024 7,145 2 View

I seed my cell in Opti-MEM?

I seed my cell (KG1a )in opti-mem media for transfection (without lipofectamine as control ) and after 2 hours I add FBS 10 ul in each wells all my cells after 24h in control wells or with...

07 July 2024 407 0 View

Do the SHSY5Y cells tend to form multiple layers over each other when highly confluent?

I have attached pics of the SH-SY5Y cells within a seeded plate after reaching >80-90% confluence. Are these dark clumps of multiple growing cell layers? Or dead cells? Any clue? many thanks.

01 July 2024 8,361 3 View

Bir Bahadur

This study may be confined to traditional rices. The Genetically modified rices rich in iron and carotene called Golden Rice may be tested as their nutritional value is high.

Aditya Banerjee

Use of seed extracts in cancer cell lines can be performed.

Carlos Adam Conte Junior

I believe that this paper can help you:

Article Scientific Evidence of Rice By-Products for Cancer Preventio...