I have used r/qtl to analyse QTL. How can I calculate the variance explained by identified QTLs in R?
In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...
01 August 2024 4,403 4 View
Hello, good morning. I would like to know which plate I can use to infect fresh PBMC with virus and culture for 24 hours. I have tried using 12-well and 24-well plates, but many cells adhere and...
24 July 2024 2,158 2 View
90% of museum objects worldwide are in storage.
21 July 2024 8,038 6 View
I'm currently working with human pancreatic organoids and I am wondering to remove the dead cells and debris from health organoids, any suggestions would be appreciated!
17 July 2024 8,330 1 View
I am on gene work to express gene of interest in some Bacillus species. Thus far, i need to try some RBS in Vector which is optimized for bacillus subtilis. Before i design vector to express in...
11 July 2024 7,847 1 View
I am a student of Mechanical Engineering. I am doing CFD opf Archimedes Winf Turbine. I am trying to validate the result of Paper. But the problem is that tha value of Power Coefficient is ever...
05 July 2024 6,839 2 View
Can I directly analyze a liquid metal-ligand sample without acid digestion in flame atomic absorption spectroscopy?
25 June 2024 1,866 2 View
While analyzing the data of point cloud, i found some strange phenomenon about the precise. But I expect there is no best plan between LiDAR and binocular vision. However, why our eyes more...
21 June 2024 5,269 3 View
For my PhD dissertation: If Chapter 1 is a systematic literature review (QUANTS/QUALS) while Chapters 2, 3, and 4 are empirical chapters (QUALS), is this considered a mixed methods research...
16 June 2024 3,220 5 View
Trouble 1: I ran a PCR reaction and found a low molecular weight band (100 bp) when doing electrophoresis. 1ng plasmid (OD260/280=1.84) was used as template. Final concentration of primers was...
13 June 2024 5,540 6 View
First, thanks for taking the time to read my puzzle 🥹! I am currently working on the methodology part of my dissertation, and I have chosen a questionnaire as the instrument for my quantitative...
27 July 2024 3,457 3 View
I have three linkage maps that needs to be integrated to create a consensus map for QTL mapping.
25 July 2024 6,505 2 View
Autonomous Language Learning can be implemented from primary to tertiary education. Practioners and students report on its effectiveness, however, there appears to be little quantitative or...
20 July 2024 2,592 2 View
Greetings! I am looking for materials on quantitative and qualitative research. What are the methods of collecting information and the principles of their implementation? I would like to make an...
20 July 2024 2,033 7 View
I am treating human cells with an AAV vector encoding for my gene of interest tagged by FLAG. I want to quantify the mRNA expression of my gene of interest via qPCR to discriminate the endogenous...
14 July 2024 8,482 3 View
What are research philosophy and paradigm and importance of quantitative research in philosophy?
10 July 2024 585 6 View
I performed a nucleic acid extraction using 2 protocols for RNA extraction (Dengue Virus) and one parameter I need to evaluate is the integrity of my RNA samples. In this case, I can't use a RNA...
09 July 2024 4,857 2 View
Pir Hassan Ali Shah post a question: How to do quantitative and qualitative data analysis after data collection? Give and review the answer in brief. All family members, friends, associates,...
08 July 2024 395 8 View
I'm analyzing abundances from various ecological surveys. So data was collected across multiple independent years from three sites within each year. The years fall under two temperature...
26 June 2024 2,798 2 View
I am currently attending MA in Community Development at Myanmar Institute of Theology, with my thesis scheduled for submission next semester. My first research used quantitative methods, and I...
26 June 2024 1,247 2 View