Hey guys, I wanna ask about my TPC (Total Phenolic Contain) calculation.
Here's what I've done
I've maserated 200g of fresh pomelo with 200 mL ethanol, then I've got my crude extract from rotary evaporation process.
I took 50 mg crude extract and diluted it with 5 mL of ethanol.
The next thing, I took 1 mL to test the TPC content.
Then I recieved my standard equation
Y= 0.001x + 0.061, with 0.082 absorbance. So I got x= 21 mg GAE/L.
Here's the question, have I done it right with this calculation 21 * 0.005L/ 0.05 g = 2.1 mg GAE/g extract?
And is it possible for me to convert it to mg GAE/ g fresh weight and mg GAE/ g dry weight (cause I've read a lot of literatures using those unit)
Thank you
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