How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Could you recommend any books about Number Theory?

Beginner level books. High level books. Analytical Number Theory. Algebraic Number Theory. Combinatorical Number Theory. Books about partitions. Books about primes.

03 March 2021 7,174 5 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

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03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Why am I getting 'Infinity' and 'NaN' in my mixed-mode energy release values?

Hi everyone, I am currently running a mixed-mode simulation of a DCB using a user-defined cohesive zone model (UEL), however, the results I am getting don't make sense. I used the same UEL for...

24 February 2021 4,157 1 View

Which miRNA arm is a right target for analysis, 3p or 5p?

I'm working on selecting mouse miRNAs to analyze and stumbling upon one confusing issue, some of the papers do not specify which arm they analyzed and they just state something like " miR-34a was...

16 January 2021 3,792 4 View

SDAS, secondary dendritic arms space relation with solidification rate?

Want to know the relation between sdas and solidification rate, the mechanism by which getting smaller space between secondary arms by increasing the rate ?

23 December 2020 735 2 View

How to perform intention-to-treat analysis for repeated measures data in SPSS?

25 November 2020 6,281 3 View

Can a DOE be used on a one-off mechanical machine bouncing in the ocean producing energy?

06 October 2020 3,761 1 View

Is it possible to measure 2 bioimpedance measurements in human body simultaneously from both the arms?

I have measured 2 bio impedance measurements from 2 AD5933 evaluation boards connected to both the arm of human body and took the readings from both the devices at once. I m getting a repetitive...

22 September 2020 928 3 View

Can a single arm clinical study validate the effectiveness of a formulation?

It has been observed that a single arm study is designed to validate a pharmacopoeial formulation for its effectiveness. As such there is no guideline available in India for acceptance of the...

21 August 2020 7,984 3 View

How to Simulate Mach-Zehnder Electro-optic modulator?

I am trying to make a Matlab/Simulink model of Mach-Zehnder EOM but I won't able to add a laser source in it and how to apply electrical data in terms of half-wave voltage to the one arm of EOM?

06 July 2020 1,660 17 View

Adding a transcriptional repressor before a promoter sequence in my plasmid?

I am performing a KI experiment using CRIPR/Cas. Our 5' homology arm is the 5'UTR of the gene of interest and therefore contains the endogenous promoter. I am looking for a repressor sequence to...

19 May 2020 5,088 3 View

Problem of custom target of board stm32f429?

Dear friend I am developing a target specific to the stm32f429disc1 card, I am following the steps described in the pages "Implement a Target for arm cortex M of the help mathworks page". I'm...

10 May 2020 9,484 7 View