I am preparing 1.4 M FA(0.9)Cs(0.1)PbI3 perovskite in DMF:DMSO (4:1). How can I calculate the stochiometric weight of each precursor materials to get this molar concentration? The precursors are CsI, FAI, PbI2.
Dear Colleagues, I have the following problem: I’m trying to amplify a cassette with a resistance gene (about 2 kb) from the yeast genome, so that I can then insert it into a plasmid. With Taq...
03 July 2024 105 11 View
Greetings, dear colleagues! Do you know any technique utilizing some ~ordinary molbiol lab reagents (not the commertial kits for isolated mitochondria staining) that will allow isolated...
07 June 2024 5,909 0 View
If Proven how Riemann hypothesis will help the development of AI and maybe help us create real advanced self-councious algorythms?
05 June 2024 9,787 3 View
We are working on gathering raw data and information about the scope/share of private donations and public funding to pilitical parties that sometimes in EU is a part of responsibilities of...
16 April 2024 5,178 0 View
I have a dataset of trace elements LA-ICP-MS analyses with raw data, which consists of a set of a single file for each spot analysis. Each of these files has some columns (masses) and plenty of...
24 March 2024 898 6 View
I am analyzing some time-series data. I wrote a script in R and used two methods from two different packages in R to calculate the DW statistics and respective p-values. Surprisingly, for the...
14 March 2024 6,127 1 View
Greetings. Probably the question is not complex at all, but can't find an answer. If I have RT-qPCR data of gene expression in a sample with multiple analitycal replicates - to compare it to data...
13 March 2024 7,640 1 View
In particular, I have few papers with spelling Agranovskii. Thank you
05 February 2024 3,764 1 View
I am using the grf R package (https://grf-labs.github.io/grf/reference/causal_forest.html) to obtain causal effect estimates for a continuous treatment variable. The package description says:...
15 January 2024 1,990 0 View
If we keep in mind that R^{4}=R^{+}xS^{3}=R^{3}*RP^{1} where * means a direct product with a singularity at the zero point of a 3-dimensional Euclidean space in which the projective line is...
30 December 2023 9,110 1 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View
Hello everyone, I need to dissolve stigmasterol for an animal experiment, but it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent for stigmasterol?
01 August 2024 7,504 2 View
I synthesised a nanocarrier by conjugation MOF (MIL-100) with folic acid for use in chemotherapy but the samples didn't dissolve completely in aqueous solution or DMSO. Instead, they only...
19 July 2024 8,522 0 View
I am conducting free radical polymerization using Kraft lignin and MMA, with DMSO serving as the solvent and there is also an initiator. Following the reaction, I obtain a solution, but I am...
15 July 2024 9,827 1 View
I'm using Dorsomorphin (C24H25N5O) as one component in dual SMAD inhibition of pluripotent stem cells to differentiate into neurons alongwith SB431542. Now as to solubilizing 10mg SB in DMSO for...
11 July 2024 4,832 0 View
I have a very polar alcohol (soluble in MeOH, H2O, partially in heated DMSO). And a long chain fatty acid that I would like to couple. I can't find a common solvent for them. I can form the...
24 June 2024 9,319 0 View
I saw the iPSC about a week after holding the stock (12 well and 3 stocks) and there were very few cells attached, and some cells were differentiated. I wonder what the problem is. The plate is...
12 June 2024 6,087 0 View
I have seeded HeLa cells in a calculation of 10000 cells per well in a 96 well plate. Then I went for treatment in the next day. Then at last, I added 7ul of 5mg/ml solution of MTT reagent into...
12 June 2024 1,460 3 View
Is it possible to do FACS on snap frozen cells ? They were isolated and directly dropped in liquid N2. They were not frozen with DMSO or FBS.
11 June 2024 8,915 0 View
Hello, I discovered that a compound that I use, which get integrated into RNA, might have an unspecific impact on mRNA stability of a gene of interest. To confirm that, I pretreated my cells for...
10 June 2024 276 2 View