A protein has 3 tryptophan residues. How can the number of buried or exposed tryptophan residues be calculated using acrylamide quenching? Elaborate on the stern Volmer equation for this quenching in detail.
I want to know more about Uranium ore deposits in world.
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I want to know more about diamond ore deposits in world.
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We assume that the difference is huge and that it is not possible to compare the two spaces. The R^4 mathematical space considers time as an external controller and the space itself is immobile in...
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If Banks do not provide credit facility, what are the options available for FPOs and impact on producer’s income?
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I used eye tracking to examine how participants from two different populations (A and B) react to an image. Participants in population A exhibit larger pupil sizes over time, but they also have...
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What are a “Farmers Producer Organization” (FPO) and its essential features?
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I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
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How do interactions between the biosphere, the carbon cycle, and the water cycle impact global warming and interaction between the atmosphere and the hydrosphere?
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I have input a moment load in module load Abaqus, i put my moment load on the node surface (using reference point). I have define moment in history output and make a set for moment too. But the...
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How is energy cycled through the Earth's climate system and how do matter cycle and energy flow through the rock cycle?
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The catalytic effect of retained austenite refers to the phenomenon wherein quenching steels with a small amount of retained austenite, when subjected to tempering at a specific temperature...
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I was performing quenching studies in solution state. While plotting the Stern-Volmer graph, which intensity should I use as initial intensity (I0). For example, I take 1 ml of 0.1 M fluorophore...
25 June 2024 5,385 1 View
Hello, I am trying to fix primary cells with glutaraldehyde. I am trying to stain cell membrane proteins and I have not been successful with PFA and methanol fixation. Does anyone have a protocol...
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I want to ask about the response of the analyte in the EIS measurement. Bare shows a high Rct response as compared to material-modified electrodes. But when we modify the material + analyte, the...
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Good evening, I was wondering what is the size of Acrylamide/Bis-Acrylamide in a PAGE 4%/8%. I'm asking because after the run I found that some of the sample stayed in the well
04 May 2024 1,566 3 View
I'm trying to remove acrylamide and lactide from the medium that didn't react to form the macromonomer
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Hi Researchgate folks, Our lab has mouse FFPE tissue which has cells that express a TdTomato reporter as well has a FAM labeled drug as we routinely use to test the delivery of the drug to the...
15 April 2024 6,632 0 View
Hello everyone, recently I am trying to synthesize a dipeptide with a Coumarin derivative of Tryptophan, and the problem is it doesn't contribute in reaction at all. Tryptophan itself in the same...
15 April 2024 6,778 3 View
I have a fluorophore and its emission is quenched on adding analyte compound. The overlapping spectra of compound and analyte make UV-vis absorption analysis unreliable. The analyte mixture...
03 April 2024 7,914 1 View
Cannot separate between 10~25kD, always have a line around 25kD... (Resolving gel buffer: 30% Acrylamide/Bis, 1.5M Tris-Cl, pH 8.8, 10% SDS, 10% APS, TEMED)
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