Why use both bead beating and enzymes (lysostaphin and lysozyme) for DNA extraction from sputum samples?

Hello, I have read in a few publications that to extract total DNA from sputum samples, both bead beating and enzymes are used to disrupt the cells. Bead beating is supposed to be efficient in...

18 February 2021 3,360 2 View

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Hello! I want to determine the activity of my in house Reverse Transcriptase enzyme. For this, I have used Thermo's Enzcheck Reverse Transcriptase assay kit. I used commercial Thermo RT enzyme to...

08 February 2021 7,285 1 View

What are the causes of hyphae granulation?

Greetings, The examination of the interaction zone between Fusarium oxysporum and Pseudomonas by light microscope revealed clear hyphae without septa and the presence of granulations (vesicles),...

05 February 2021 5,523 4 View

Which software should be used for XRD analysis in case of soils?

I am using XRD test for indentification of minerals and amount of it.

10 December 2020 8,173 4 View

How to improve total RNA concentration after transfecting the cells with the desired delivery system ?

Hello, I have been doing some transfection experiments for certain gene studies and wants to further do gene expression studies using qRT-PCR. Currently, I am using 7*10,000 cells per well for...

08 December 2020 6,981 5 View

How to decrease the difference between the simulation result obtained by comsol and analytical result ?

Hello, I am working on a piezoelectric energy harvester in comsol so I coupled the electrical circuit interface with the piezoelectric device multiphysics. I tried to confirm these results with...

07 December 2020 1,007 2 View

What is the index of germination and howa calculate it??

16 November 2020 4,856 8 View

How do I determine the units of polymerase activity for my enzyme DNA Polymerase?

One unit of DNA polymerase activity is conventionally defined as the amount of enzyme that will incorporate 10 nmol of nucleotides during a 30-min incubation. I found out about the...

04 November 2020 8,824 7 View

Hormone levels in male mice ?

Dear Colleagues, Can anyone tell me if there are Data about hormone levels in male mice , E2 Estrogen, and Testosterone blood levels in male mice. Thank you very much.

20 September 2020 1,035 3 View

How can I fix the connvergence error!?

opt freq gaussian calculation is performed, I got normal termination , however, the structure has nottotaly converged. because I had no convergence in Maximum Displacement! how can I solve this...

06 September 2020 4,283 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Samples to study circular dichroism (chirality)?

I want to build a low-cost polarimeter to study circular dichroism (chirality). What type of samples can I use? I am looking for samples that can be found outside the laboratory.

01 March 2021 9,101 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Can anyone help with Schistosoma identification?

Can anyone identify what kind of Schistosome species this might be? Morphological features of cercariae and adults resemble Trichobilharzia, though I am fairly new to the field of parasitology...

25 February 2021 7,760 6 View

Suppose we have a banana seller cart consisting of 4 wheels, weighing about 300 kg. How much torque motors are needed to pull of push it electrically?

I am thinking of electrifying the street food carts while harvesting energy using solar panels on top.

22 February 2021 4,323 3 View

Does anyone have any experience with the Motic EasyScan Pro Slide Scanner?

I am interested in purchasing a slide scanner for histology and was presented with the Motic EasyScan Pro but I have never heard of the company, nor know of anyone who has used the system. Can...

11 February 2021 2,416 2 View

Translating two biomarkers to the same scale??

Hello researchers, I was wondering if anyone had experience in the following: I would like to translate two separate continuous biomarkers (A and B), that are mechanistically linked to each...

25 January 2021 2,107 3 View

What time and speed should the centrifuge be set at in order for cell debris and clumped proteins to form a pellet?

- The Eppendorf tube was then placed in a microcentrifuge. Notice how it is balanced with another tube placed opposite it in the centrifuge drum to ensure smooth rotation. Other information...

24 January 2021 2,547 3 View

Is Handheld XRF analyzer useable? and accurate in geochemical test?

What is the different between Lab XRF and Hanheld XRF, is it accurate and useable to analyze sedimentary rocks?

23 January 2021 9,941 6 View