Hi,
I´m starting to work with bacteriophages to control Vibrio parahaemolytcius responsible of acute hepatopancreatic necrosis disease in shrimp
The MOI is not something you calculate but something you adjust to. You should know the number of cells you are adding and the titer of your phage stock, so you select the MOI you want to have and adjust the volumes of phage added to the known number of cells to reach that MOI.
For EOP that is a relative value to some known mechanism of counting your phage. So you may have a good host and you know your phage titer is 1e8 for example. And then you plate on a different host and you get a titer of 2e7. So the EOP would be 0.2 or 20%. But it is calculated relative to some known control value.
Thanks a lot Michael, do you know an easy guide to learn how I do that?
What are you trying to do precisely? Because I’m not sure what you asked about is really what you are wanting to do.
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