Is it possible(or logical) to notice an elevated autophagy using LC3-II based immunoblotting, but no difference by using GFP-LC3 based imaging study?

For example, I treat cells with "X" - performed immunoblotting for LC3-II - found elevated autophagy, then did the same treatment in GFP-LC3 transfected cells followed by confocal imaging - found...

06 April 2020 4,332 4 View

What is the zeta potential value of deionised or distilled water?

I am getting zeta potential value of about -30 mV for distilled water obtained from a commercial supplier. What it means? What should be the zeta potential value of distilled water? How can I...

10 October 2017 2,791 19 View

Dear all, can anybody suggest me how to seperate phytoconstituents from alcoholic extract of plant material using column chromatography

I successively extracted a plant material with different solvents, among these alcoholic extract shown best activity, for this I did GC/MS analysis. further I would like to separate the...

09 August 2017 7,760 2 View

How to calculate refractive index from Uv visible spectrum?

send the link i.e formula

11 June 2017 6,371 10 View

Will the natural convection ( CFD simulation) problem within an enclosure converge thermally?.Because there is an external solar load as well?

I am solving the natural convection heat transfer problem within an enclosure with few heat generating components using Fluent.Around this enclosure,I have considered the fluid domain. To consider...

17 November 2016 6,131 5 View

Zeta potential for amine functionalized Nanoparticles?

What will be the sign of zeta potential for amine functionalized nanoparticles? The amines are neutral (not protonated). Due to the electron rich character of amine functional group shall I expect...

30 July 2016 9,866 4 View

Does anyone know about "Asymptote" Software and online learing?

25 June 2016 4,421 2 View

How to calculate refractive index of small size crystal??

24 June 2016 4,039 1 View

Is the zeta potential value valid for amino acid functionalized nanoparticles?

I have modified my carbon nanoparticle surface with amino acids. Now I would have both amine and acid functionalities on the surface of my nanoparticle. The measured zeta potential value is about...

31 December 2015 6,592 10 View

Why do I observe a downregulated gene in RNA-Seq that is upregulated in qPCR?

15 November 2015 4,108 4 View

Why Austenite grains in steel show much more twinning than ferrite grains?

It is oftentimes said to be a distinctive metallographic trait that differentiates a fully austenitic and a fully felly ferritic steel without etching that might distinguish between two. Why...

02 March 2021 728 2 View

Is there a deep reason for using the squared modulus of the wave function as the probability density of the existence of the particle?

I think we were all surprised at the first time we got to know quantum mechanics that the squared modulus of the wave function is the probability density of the existence of the particle? The role...

28 February 2021 6,282 9 View

Why am I not able to use all VEP programs for the in silico analysis of avpr1b SNP's?

Good day researchers I am busy analysing the predicted effects of certain SNP's in AVPR1b. When using the VEP prediction programs (SIFT, Polyphen, FATHMM, LRT, Provean, Mutation Assessor, and...

28 February 2021 5,197 1 View

What is the difference between genotype and haplotype data in the ped format?

Hi everybody In the ped format for genotype, alleles of any SNP are represented by two columns (one for each allele, separated by a space). Is a column sufficient for the haplotype to...

27 February 2021 1,965 1 View

Why do the air flow results vary totally when adding scalar in CFD ?

Dear All, We are using Autodesk cfd to calculate the air flow in a single sided naturally ventilated room, where air flow depends only on buoyancy due to temperature difference between inside and...

25 February 2021 9,602 4 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

How to search for crystal structure for material with atomic position labelled?

I am using the crystallographic open database and materials project to search for cif file but the exact structure is not mentioned or how can I deduce that the given structure and atomic position...

23 February 2021 6,723 2 View

How to generate AA6061 alloy or any aluminium alloy crystal in material studio?

I need to carry out molecular dynamics simulation on Aluminium alloy. For this i need the crystal structure that which could be further replicated to form a structure.

22 February 2021 1,410 1 View

Is there a suitable way to confine cell attachment area inside a multi-well plate?

Are there any types of cylindrical cell culture inserts that are designed to confine cell seeding area? Say, if I'm using a 12 well plate (3.5 cm^2/well), but only want to seed cells in the middle...

22 February 2021 7,127 4 View

Build an orthogonal box in ASE?

Hello, I was wondering the best way to build an orthogonal box filled with a defined crystal structure in ASE. I know it could be easily done in LAMMPS, but I guess it is also doable in ASE. The...

21 February 2021 5,899 4 View