Is it possible(or logical) to notice an elevated autophagy using LC3-II based immunoblotting, but no difference by using GFP-LC3 based imaging study?

For example, I treat cells with "X" - performed immunoblotting for LC3-II - found elevated autophagy, then did the same treatment in GFP-LC3 transfected cells followed by confocal imaging - found...

06 April 2020 4,332 4 View

Effectiveness of Fiscal policy ?

Hi all I am about to start a research / Dissertation in the area of fiscal policy in Australia. More specifically I Would like to start off with a Dissertation at Masters level leading to a...

04 November 2019 7,491 3 View

How to interpret the effect of wear after Pin on disc test using SEM?

what is the purpose of taking SEM image after Pin on disc test,if so how to identify the wear mechanism.

09 May 2018 4,630 4 View

'DIFFERENCE' not defined in SetByBoolean

17 July 2017 559 3 View

Would you please give me the receipe for BSTS(topological insulator) thin film etching

Or please suggest papers from where i can find the same

04 June 2017 3,191 1 View

How to calculate refractive index of small size crystal??

24 June 2016 4,039 1 View

Does anyone have a Nav1.6, 1.7, Nav1.8 and nav1.9 plasmid to express in HEK cells?

02 March 2016 9,114 1 View

Clone having repetitive sequence insert and its discripancy upon scale up?

I have cloned my 3.3Kb insert having repetitive sequence in yeast expression vector, by directional cloning in E.coli. Positive Clone of 8.7Kb was confirmed by restriction analysis. While the same...

26 January 2016 4,477 2 View

I am facing a problem in binding and elution of an MBP tagged recombinant protein from MBP column. Can anyone lend their expertise?

I use 5X 0.5M HEPES buffer and 100mM ZnSo4 Nacl buffer into 1x as column buffer with pH 7.4 and 1X column buffer with 0.5M maltose as an elution buffer. Please suggest how I can overcome this...

15 April 2014 8,300 2 View

Does poverty and ignorance contribute to a danger for nature?

One source of poor peoples livelihood comes from wood firewood selling, indirectly loss of forest, which bring the danger to nature.

19 March 2014 8,378 70 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

How to arrange coordinates and extract climate dataset out of MPI-ESM-MR netcdf files?

Have a nice day everyone, I'm stuck in extracting datasets out of MPI-ESM-MR netcdf files because the latitude ranges from -3*10^6 to 3*10^6 and longitude ranges -5,9*10^6 to 5,9*10^6. Plus, the...

28 February 2021 912 3 View

Is there any Machine Learning/AI algorithm that lets you predict with incomplete features without missing value imputation process?

For instance, the model is trained with A,B,C,D,E features to predict F target. Then, I want to make prediction on a new data, but with only A,C,D,E is known without doing missing value imputation...

28 February 2021 1,556 6 View