I'm culturing micro algae strains and need to monitor the growth. We are trying to do a comparison of the different methods to obtain cell count. The use of absorbance to determine cell count is a popular method. Could somebody help me with a formula to calculate the cell count using a spectrophotometer?
Thanks
Dear Shaima,
You can consult the paper entitled
"Direct spectrophotometric method to determine cell density of Isochrysis galbana in serial batch cultures from a larger scale fed-batch culture in exponential phase" by Gomez et al. (2016).
In general practice the absorbance is measured in between 420-660 nm and depends on the absorbance of the materials investigated. You can either use McFarland Turbidity Standards using Barium Chloride and Sulfuric acid or using pigment absorbance, you can delineate your reference standards from the duplicate cultures following manual counting of the cell density from aliquots taken. As spectrophotometer blank you can use preferably the culture media in which the cultures are to be grown. The length of the path of light to be traversed i.e.the cuvette or cell diameter also determines the final output significantly.
im growing scenedesmus sp., nannochloropsis sp. and chlorella sp.
Through a curve of Absorbance /density -- with a lot of bench mark data
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