While performing SDS page (for microbial crude enzyme) several times, I could not get any visible proteinbands on the gel. What might be the reason?

I am wroking on microbial enzyme and after its extraction in crude form, I had performed the SDS PAGE. But I was not able to get any visible band of protein instead of repeating the whole...

04 March 2020 1,956 4 View

How can you interpret the true meaning of 'Development'?

'Development' itself is a confusing word as it indicates several dimensions altogether. I wish to know the true meaning of 'Development' which possess a holistic acceptance. How can I designate a...

07 January 2020 1,188 3 View

Is organic farming the last alternative left for the future food security?

Our contemporary world is moving towards an uncertain food insecure period and an invisible famine is rather approaching towards us to grasp the lives. So, how this problem of food insecurity...

02 November 2019 9,625 24 View

Is farmers' destitution exactly inescapable in the global agricultural scenario, especially in the third world?

Farmers are always in the poverty mostly in the developing world. Their poverty is somewhat getting stacked with their lives despite various schemes & farmer welfare programmes. Could anybody...

20 October 2019 2,781 3 View

What are the differences between reaction by hydroxylamine.HCl and o-methoxyamine HCl. Which one is better in oxime formation reaction?

24 November 2016 2,245 3 View

How can I get authenticity for my alkaloid purified ?

I have purified alkaloid from papaya leaf extract. I underwent IR for the same. They are in good accordance of peaks. As a result i performed confirmatory chemical test for all functional group....

11 May 2016 3,349 6 View

Hi, I want to overexpress a gene named SPRY4 in a transfectable cell line. Can anyone recommend me any easy and effective way to do that?

16 November 2015 2,648 2 View

Is representation theory an essential component of a rigorous graduate course in general relativity?

I have been observing the connections of differential geometry, representation theory and lie theory in general relativity, and was wondering what's essential in the view of a course on general...

16 March 2014 3,386 6 View

Can someone suggest a software simulator for vertical axis wind turbine blade design?

I want to optimized my basic design of a new kind of VAWT blade.

15 January 2014 8,548 6 View

How does K shell electronic wave-function change at the position of nucleus, when K X ray is emitted?

When a K shell X ray is emitted due to transition of electrons from upper states to K shell vacancy, is there any change of electronic wave-function of K shell at the position of the nucleus? Any...

16 December 2013 4,833 3 View

What are anisotropic electrical and optical properties of a semiconductor due to?

In some materials, properties such as band gap, thermal conductivity differ along different crystallographic axes. What are the basics?

01 March 2021 1,923 3 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View

What information can be had from raman at higher wavenumber?

i have recorded raman spectra of strontium oxide thin film. the spectra were recorded in the range 400 to 4000 cm-1. i have reference to peaks upto 1000cm-1. but i couldn't find literature for...

26 February 2021 2,499 1 View

Is it possible to have two luminescence bands of the same peak position but with different intensities in single spectrum??

Hi! If it is possible to have two luminescence bands of the same peak position in a spectrum, what is the reason behind formation of two separate bands instead of one? This phenomenon is related...

26 February 2021 4,963 5 View

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25 February 2021 5,713 1 View

How can I find a Raman spectrum wavenumber corresponding to what compound?

I have several Raman spectra with very distinct peaks at certain wavenumbers, such as 277, 359, 466, 583, 623, 686, and 795cm-1. How can I identify them to what compound(s)? The RRUFF and others...

24 February 2021 363 3 View

How to prepare a thin polymer film by post-polymerization/crosslinking?

24 February 2021 2,362 5 View

Is is possible to pole with corona poling pellets of the sample material instead of thin films?

As it is highly probable that pellets will have a higher degree of air gaps and voids than thin films, is it possible to pole the samples with corona poling nevertheless? Or is there the...

24 February 2021 1,349 1 View

How to troubleshoot Electrochromic Films and Electrochromic Devices?

I am trying to characterize an electrochromic system. The system uses ITO electrodes on glass, WO3 as the electrochromic film, and NiO as the ion storage layer. When I deposit NiO and WO3 and...

23 February 2021 9,592 2 View

What are difference between laser diodes, simple light emitting diodes and power light emitting diodes when operated in the visible spectrum ?

I want to illuminate a scene (small space) using LED to capture photographs or videos of some tiny objects inside the space. But am not sure if I should use a Laser LED, or simple LED or power...

22 February 2021 4,240 4 View