Could research community help me to sort out the problem related with structural equation model(SEM)?

I am trying to examine the impact of cognitive and psychological factors, socio-demographic factors, Experimental processing factors and socio-cultural and institutional factors on farmers risk...

14 May 2019 4,341 1 View

Can anyone help me out with a protocol for cell phagocytosis assay where I wish to use U937 cells for phagocytosis of cancer cell.?

I wish to optimize a protocol where I need to see the phagocytotic activity of U937 against cancer cell. Please help me with this. It will also be a big help if you can suggest me some papers.

04 February 2019 5,319 1 View

A follow up from the previous question, can you suggest papers where U937 cells are treated with IFN-gamma?

I have treated U937 cells with IFN-gamma for FcyRI induction but unfortunately could not get any induction. Can you let me know about papers where they have shown this experiment?

02 January 2019 781 1 View

Which is a better way for determining ROS production?

I wish to estimate ROS production using U937 cells. I have found some papers using Lucigenin for the chemiluminescent assay and some papers using DCFH-DA. Can you please let me know a solution for...

01 January 2019 9,069 13 View

Major consequences of stress?

The research studies has confirmed that stress has major consequences towards the health of a person... If a person is having a continous stress for a month he/she is possible of getting diabetes...

31 August 2018 1,759 6 View

List some fast food related journals to publish an article which is related to fast foods ???

Presently I am in research project related to fast foods like pizza hamburger and so on kindly suggest any good journals...

21 August 2018 6,258 6 View

What is composite analysis in meteorology and what are its importance?

What is the main purpose to do Composite analysis in meteorology?

08 May 2017 646 12 View

How to apply Load normal to a node in ANSYS APDL

I am new to simulation in ANSYS. I wan't to know how to apply Load normal to a node in ANSYS APDL. I am attaching the pic of the model.Please help me in applying load normal to any node.

11 March 2017 7,659 1 View

No title

26 October 2016 3,552 5 View

What would be polarity of methacrylic acid and EGDMA, can they be used as solvent?

methacrylic acid is used for preparing polymer for molecular imprinting, can it act as polar solvent along as a substrate.

04 October 2016 3,534 2 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

For modelling epoxy-based matrices (in composites), which model in ABAQUS is the most suitable, Concrete damaged plasticity or Drucker-Prager model?

I am working on developing a micromechanical FE model for predicting kink-band formation in UD composites. To model matrix plasticity, which model, out of Concrete Damaged Plasticity and...

28 February 2021 5,415 1 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

Quantifying leaking DNA from bacteria - what's the secret?

Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...

24 February 2021 2,006 2 View

How the Ground Plane implantation is performed without damaging (and w/o varying the undoped nature) the top Si (8-10 nm) in FDSOI technology ?

It is a general practice to introduce Ground Plane in FDSOI devices under the BoX. The Ground Planes are introduced by Ion Implantation through Top Si layer and Buried Oxide. I wanted to know that...

24 February 2021 5,974 4 View