Gargi Nanda

4 Questions 17 Answers 0 Followers

Questions related from Gargi Nanda

How to calculate %Tail DNA for Fluorescent stained comets in CellProfiler?

Hi, I ran the CellProfiler using the ExampleFluorescentCometAssay.cppipe pipeline. However the output .csv file for comet tail do not show %tailDNA for the analysed comets. Is there a module that...

29 November 2016 639 2 View

What if allele frequency is not associated but the genotypic frequency is associated with a disease?

I have problem concluding this data of polymorphism association with a disease. The SNP shows no significant association with respect to (wrt) allelic frequency (P >0.05) but shows a marginal...

28 September 2014 1,430 7 View

Can you troubleshoot my Simple Allele-discriminating PCR (SAP)?

I'm trying to perform SAP for a particular SNP using usual Taq pol (not hifid) and primers designed using WASP (http://bioinfo.biotec.or.th/WASP); as suggested by the authors (Bui and Liu). But...

22 September 2014 4,414 10 View

What is maximum limit of gene size to clone into Drosophila?

I want to clone a gene of 413kb size into drosophila system. Can anyone suggest the success rate of transformation of such sizes of genes and also what is the gene size limit for transformation in...

16 September 2014 7,542 7 View