Hello, I'm trying to calculate the standard error in order to calculate the 95% confidence interval to represent error bars on a graph and I am stuck on the calculations when using RPKM. The data I have is from a colleague's RNA seq and I'm unsure how to do the calculations. I would appreciate any help I can get, thank you!
The data is as follows:
Total linear RPKM
R1 R2 Fold change pvalue
Control 8.82 1.85 2.24 0.622
Sample 1 355.63 245.66 164.46 0.049
Sample 2 10.42 11.49 6.09 0.26
*Note R1/2= Replicates