Areeg Abd Elhafiz

4 Questions 5 Answers 0 Followers

Questions related from Areeg Abd Elhafiz

How to calculate standard error from RPKM replicates?

Hello, I'm trying to calculate the standard error in order to calculate the 95% confidence interval to represent error bars on a graph and I am stuck on the calculations when using RPKM. The data...

12 December 2021 1,221 2 View

Does anyone have a protocol to identify protein-protein interactions in Drosophila?

Ive just made a tagged over-expression line of my gene and I want to identify any protein-protein interactions. Does anyone have a protocol I can use, the procedure in my lab is to use Brain ring...

07 June 2021 3,004 3 View

Has anyone attempted cDNA synthesis followed by qPCR on a RNA sample with a low 260/230 ratio of 0.3?

I've recently completed a bunch of RNA extraction and noticed a 260/230 ratio ranging from 0.3-1.17 indicating possible phenol contamination. My 260/280 ratio is relatively good 2-2.1, this is my...

10 May 2021 9,233 3 View

Confusion about Antibiotic calculation and dilution factor question?

Im really bad and math would like some clarification, on addgene it states "to dilute your antibiotic into your LB medium at 1:1,000. For example, to make 100 mL of LB/ampicillin growth media, add...

14 April 2019 7,890 1 View