Hello dear researchers!

We have been trying to calculate amount of spores in spore suspension of Metarhizium anisopliae by hemocytometer. However, hyphae get on our way as it disturb the calculation and not to mention the presence of clumpy spores. We have tried a few methods such as filtering the spore suspension through double cheesecloth as well as by adding 0.05% Tween 80. Nevertheless, to no avail. Any ideas are much appreciated. Thank you.