To measure the proline of Bates et al (32) was used. Based on this method leaves 5/0 g of each sample in 10 ml of an aqueous solution of acid Solphosalisilic 3 percent, and the mixture is completely homogenized in the Chinese Havn. Then the mixture was homogenized by Whatman filter paper (2) is clear. Then, 2 ml of this solution with 2 ml of reagent creatinine dimenhydrinate (1.25 g creatinine dimenhydrinate for this reagent in 30 ml and 20 ml phosphoric acid, acetic Lytrasyd 6 M solution) are mixed and 2 ml of acetic acid is added to each tube. Samples for one hour at 100 ° C water bath with shower, and immediately out of the bathroom for a few minutes to put ice bath. After this step 6 ml of toluene added to each test tube samples with stirring for 15 to 20 seconds are stirred up quite uniform. The pipes are placed for some time in the laboratory. During this period in a test tube 2 upper and lower phase completely distinguishable and the upper phase according to the standard curve to determine proline and proline in a spectrophotometer at a wavelength of 520 nm is used.

I know method...thx....i have problem in calculation part..

Dear Soni

It is calculated based on amount of tissue that you use for this protocole.

Hey Priyanka Soni ,

Did you figure out the calculation part? I had confusions with the volume of toluene being considered and that sulfosalicylic acid and glacial acetic acid being not taken into account? Is it correct if we simply use 4ml in toluene?

Thanks