I am studying the release for 96hrs. How do I calculate the percentage drug release? I have plotted the standard curve and r2=0.998.
First Step
Determine concentration of drug released by using formula
Y =mX +/- C
Y is absorbance, m is slope, C is intercept, X is concentration (mcg/ml)
Second Step
Calculate amount of drug released
Amount of drug released (mg)= [Concentration X Dilution factor X Volume of Dissolution medium] / 1000
Final Step
% Drug release = Amt of drug released (mg) X 100 / Dose (mg)
First Step
Determine concentration of drug released by using formula
Y =mX +/- C
Y is absorbance, m is slope, C is intercept, X is concentration (mcg/ml)
Second Step
Calculate amount of drug released
Amount of drug released (mg)= [Concentration X Dilution factor X Volume of Dissolution medium] / 1000
Final Step
% Drug release = Amt of drug released (mg) X 100 / Dose (mg)
Formula for determination of percentage of release of drug from in vitro dissolution testing
- Concentration of drug (µg/ml)= (slope × absorbance) ± intercept
- Amount of drug = Concentration × Dissolution bath volume × dilution factor
released mg/ ml 1000
- Cumulative percentage = Volume of sample withdrawn (ml) × P (t – 1) + Pt
release (%) Bath volume (v),
Where Pt = Percentage release at time t
Where P (t – 1) = Percentage release previous to‘t’
Use UV-VIS spectrophotometry and Buger -Beer law: D=e c l, where D-absorbance in arb.units, e-molecular extintion coefficient in l/mol cm, c-concentration, Mol/l , l- cuvette thickness, cm. Wright down spectra of your solutions and measure D max of suitable spectral band (do not forget to measure and substract background). e is measured as a slope of the linear plot D = f(c) for 4-5 points (concentrations). The concentration in Mol\l recalculate for your volume and multiply by molecular weight, after that you will have the quantity of your substance in grams. Divided it into initial content of this substance in the matrix and multiply by 100. You will have result in %.
first step : calculate of the release concentration(Ce) from calibration curve at any time
second step : calculate the amount drug release = Ce*V(L)/m
third step : calculate percentage drug release = amount drug release / amount drug loading *100
Thanks all. I have really learnt. I am hoping the dose in Mr. Kailas equation means the theoretical total quantity of active ingredient in the tablet.
Dose is strength of tablet (Total quantity of API).
Check attached file
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