I weighed 10g of fish samples then homogenated with 100 mL of solution [ 92.5ml distilled water + 2.5 ml HCL]. After homogenization and filtration, 5 mL of the liquid extracted was mixed with 5 mL of TBA solution. Then the solution was heated at 95°C and after cooling Abs was recorded.
A calibration curve was plotted with 1,1,3,3-tetraethoxypropane (TEP) and the slope was recorded.
Hi Vince
This question was also asked previously on Researchgate. Have a look at these replies and hopefully they will help
www.researchgate.net/post/1_1_3_3-tetraethoxypropane_to_malondialdehyde_conversion3
best wishes
Hi there!
Usually lipid oxidation values are displayed as mg MDA/kg sample or mg TBARs /kg sample, could be mg or other units accordingly to method used. To transform the values from the TEP standard curve to these units, there is a factor, called K factor. It uses data from you standard curve, concentration, recovery and goes on. I recommend read the following article:
Evaluation of the 2-thiobarbituric acid method for the measurement of lipid oxidation in mechanically deboned gamma irradiated chicken meat (Food Chemistry, v. 80, p. 433-7, 2003)
Heliana de Azevedo Gomes, Edir Nepomuceno da Silva, Marcos Roberto Lopes do Nascimento a, Henrique Takuji Fukuma
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