How to calculate limit of detection of biosensor from calibration plot ((F0-F/F0) vs -Log Concentration)?

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Dalibor M. Stanković

yes you can calculate Sb and from that you will obtaine some value for -log concentration. After that recalculate back to have concentration. it is similar how you will calculate unknown or detected concentration from calibration curve

Mohammad Nateghi

This is exactly same as direct potentiometric measurements based on Nernst equation (which is a semi-log relation between electrochemical potential and logarithm of concentration).

António Maximiano Fernandes

S/N=3

S: Lowest signal

N: Base line signal

y=mx+b

y=signal of lowest concentration

x=lowest concentration analyte

Hassanein Shaban

Article Detection Limit Estimated from Slope of Calibration Curve: A...

Reza Karimi Shervedani

باسلام

Dear Clleague

Have a look. Should be useful.

https://chemistry.stackexchange.com/questions/141369/detection-limit-for-log-log-calibration-curves

موفق باشید

Good luck with your activities.