Question: How to calculate/evaluate Joint toxicity test of two compounds namely: A and B in an aquatic system for zebrafish? The conditions are the following:
1. Both the compound are liquid and soluble in each other as well as water
2. LC50 of compound A (gm/L): 24 hr- 4.46; 48 hr-4.07; 72 hr-3.89; 96 hr- 2.45
3. LC50 of compound B (gm/L): 24 hr- 8.12; 48 hr-5.24; 72 hr-4.67; 96 hr- 3.54
4. How can we say the Chemical interactions between the compound in an aquatic system are showing (a) antagonistic effect (b) additive effect (c) synergistic effect.