Can we solate viable cells from frozen xenograft tissues?

I am planning to isolate cells from frozen xenograft tissues (stored in liquid nitrogen). The tissues are stored in 10% DMSO, 30%FBS and 60% RPMI with Penn/Strep. These tissues were frozen with a...

14 December 2020 3,910 1 View

What can i do to extract antimicrobial compound from bacterial medium supernatant if do not know the nature of antimicrobial compound?

I am having an unknown bacterial hit so far, which is inhibiting the growth of multidrug-resistant bacteria. I want to characterize that antimicrobial compound.what should I do to characterize...

21 September 2020 6,869 3 View

How to identify fake research and predatory journal?

Fake researches and predatory journals

14 September 2020 4,530 7 View

Hello Everyone, Can anyone help me in the transfection procedure of plasmid into chicken spermatozoa by electroporation?

Hello everyone, I am doing an experiment in which, the plan of work is to electroporate a clone (plasmid form) into chicken spermatozoa, which will be used further for artificial insemination....

07 September 2020 7,855 2 View

CG relaxation with B3LYP in VASP?

I am trying to CG relax a amorphous GeSe system with B3LYP and single K-point (gamma). Even though I have a 250 atom model, the calculations seem to be extremely slow. Is there a way to speed up...

23 August 2020 8,848 4 View

For Scanning electron microscopy based trichome analysis of the leaf samples fresh samples can be preserved?

For SEM-based analysis of trichomes (soybean plant) can we preserve the samples collected for some period of time? or after following standard fixation procedures it can be preserved under low...

01 June 2020 5,665 1 View

New problem Statement on Solar Energy?

Dear All, I want to know the new research topic on Solar Energy for my PhD. work. Please let me know.

13 January 2020 4,388 1 View

Can I use XBridge C18 ( 3x150mm, 3.5 um) column in a Aquity UPLC system for method development?

I have a UPLC system and XBridge C18 ( 3x150mm, 3.5 um). I am running short of time and cant procure a UPLC compatible column for the time being.

15 December 2019 8,433 12 View

Can anybody share links of dorsal hand vein images data set to download?

I need a data set of dorsal hand vein images for experimental purpose.

07 October 2019 1,438 1 View

How to dissolve PtCl2 in water?

HI, I am finding difficulty with the solubility of Platinum (II) chloride. I wanted to prepare PtCl2 in water for electrochemical application.

21 August 2019 5,444 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

Irradiation data for JAPAN?

Hi, Could you help me to get the latest (2019, 2020) solar resource data for a particular site in JAPAN? PV GIS and NSRDB data are not in reach to JAPAN, I'm looking for some other database for...

03 March 2021 961 3 View

Does anyone know to clarify these changes?

01 March 2021 1,290 2 View

How can I make stacked bar graph with scatter plot?

I want to make a stacked bar graph with showing each data value as dots. I tried with graph pad prism, version 6, but could not succeed. Does anyone have tried before making such graphs?

01 March 2021 9,383 1 View

How to Eliminate cosine error and abbe error in LASER measurement system(for example Rhenishaw XL80)?

I like to do project in laser measurement system. My objective is to eliminate or reduce the cosine error, abbe error in Laser measurement system. I have Rhenishaw XL80 system. Please tell the...

28 February 2021 4,890 3 View

How to calculate the crystallinity of cellulose film?

27 February 2021 2,753 4 View

How to calculate Ligand-active site distance in a ligand-protein complex from MD simulation?

I have run a MD simulation of a ligand-protein complex in GROMACS using CHARMM forcefield. Reviewer has asked me to calculate Ligand-activesite distance and binding free energy of ligand with...

27 February 2021 4,703 1 View

What could be the reason for not having further low chi square value (better agreement) in my refinement result?

I have tried every combination of parameter changes and after that i have a poor chi square value. I can't get it lower any further. I know there have been a lot of discussion about Rietveld...

26 February 2021 8,480 3 View

Can anyone share his experience regards Smoothing in origin?

I am working on TiO2 composite material and the results of XRD have a lot of small/junk peaks and while I apply a smooth filter in Origin then the other characteristics peaks disappear so anyone...

26 February 2021 8,183 3 View