Hi dears,
My question is about encapsulation efficiency of silymarin-loaded nanoparticles.
As you know, the equation is as below:
EE% = Total amount of extract - Surface amount of extract / Total amount of extract.
I have not detected silymarin concentration by HPLC, so in order to get the surface and total amount of silymarin and EE%, I've used total phenol value detected spectrophotometrically.
Is it a correct way?
thanks
What you need are the initial and final concentration of Silibinin in the medium. No matter what the method you use for measuring the target molecule concentration.
Dear Mr. Sepher, please consider Ultrafiltration method. For nanoparticles it is mostly applied and it is done with Amikon ultrafiltration tube. The unbound particles will settle down in the tube after centrifugation and you can measure Silymarin content in that and then use it in the formula to calculate EE.
I also wonder about one thing, I believe Silymarin is an extract with a mixture of active ingredients. So it is difficult for you to detect a single peak, were you able to get a single peak? If so, what would be its active ingredient?
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