Initially, I added a known conc of internal standard (IS) in my saturate fraction and then integrated the area of the internal standard from the GC-MS and from this calculation I easily callculated the conc of C17 in my fraction.
For example, Conc. of C17 =[ IS conc. X C17 area]/IS Area.
Please tell me is this procedure accurate? I followed some books where it has been asked to use calibraton curve. I really don't understand what's the point of using calibration curve to find the unknown conc. of any carbon compound in my sample. Please help me out. Thanks in advance.
Please read the attached paper
I think it will be helpful to your calculation
Mr. Patra,
Please, focus your attention on the following discussion, where I have presented our own-authored (mine and my co-author's theory according to the athorship of teh papers) stochastic dynamic theory and model formulas for data-processing of mass spectrometric variables, yielding to exact or absolute quantification of analytes.
[https://www.researchgate.net/post/How_to_develop_HPLC_method_for_D-Fructose]
In particular, please, concentrate on the reference section, therein.
Despite, that the topic of the discussion is on analysis of carbohydrates; our approach is applicable to any analyte types.
The quantification within this theory can be carried out without internal standard.
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