Post evaluation of close range photogrammetry experiments ?

I am performing a simple close range photogrammetry test and comparing outcomes of photogrammetry tools such as VisualSFM, Meshroom, Colmap etc. Kindly advice, what post evaluation tests/ criteria...

13 February 2021 6,410 3 View

How to define two phase change materials with different operating (boussinesq) temperature in cell zone conditions of FLUENT?

Hello, i am trying to simulate two PCMs in a domain. I assigned their material properties in materials tab. However, when i go to cell zone condition, i can only put one value for boussinesq...

04 November 2020 3,943 3 View

Can we use PEG 400/600 in Dissolution medium, at what level of % ?

Surfactant aid in release of poor soluble drugs but in some cases discrimination is compromised. Can we use PEG if it gives better results in terms of release and discrimination power of...

21 September 2020 6,907 4 View

What is the best way or source to search for the Innovator of a drug product?

In Pharmaceutical professional fields such as Regulatory Affairs or Product Development it is of much importance to know the innovator of a product for obvious reasons. But it is not a very simple...

26 April 2020 7,475 1 View

NH4:NaOCl mixing ratio of 3:1 is mole ratio or the mass ratio?

18 March 2020 6,847 1 View

How to make nutrient broth to grow rhizosphere bacteria in it? It will be good if i get a robust protocol?

i want to grow rhizosphere bacteria on nutrient broth and subsequently check their growth through OD. I need a robust protocol that can tell me all the details about conducting this experiment.

23 January 2020 4,734 3 View

How to calculate the cross polarization discrimination when given a 2x2 channel of a dual-polarized antenna?

If I have a 2x2 channel matrix with all channel coefficients being complex. I am using this model for a dual-polarized antenna. In order to use this model, the XPD should be calculated which is...

23 December 2019 1,568 4 View

What could be the possible reasons of non-significant G x E interaction across normal and late sowing date?

I have conducted an experiment on wheat crop to observe the response of different traits under normal and late planting dates.

27 November 2019 480 3 View

Black and white area percentages in a binary image: MATLAB vs imageJ?

I was wondering which of these two is a better tool for black and white area percentages in a binary image analysis i.e. MATLAB or image. Assuming the image is of an intricate network of a porous...

20 May 2019 8,972 1 View

What is the impact of green leadership on organization performance?

how the green leadership impact the organizational performance toward achievement of sustainability.

16 May 2019 2,666 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Why initial concentration of Pb not the same as calculated one when testing using AAS?

I am currently doing research on the adsorption of heavy metal using magnetic reduce graphene oxide. I use AAS to determine the concentration of the heavy metal. I prepare the Pb solution with...

26 February 2021 9,580 4 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View