How to calculate absorption correction coefficient for FullProf?

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How to activate 4E8 T cells with plate-bound anti-CD3 for a genome-wide CRISPR screen?

It was assumed that 4E8 T cells should be isolated and activated using plate-bound anti-human CD3, after which would be used for a genome-wide CRISPR screen. (Ref: Genome-wide CRISPR Screens in...

17 July 2024 3,371 0 View

Is there any protocos to produce the medium for single hybridoma cell culture without the feeder cells layer in the plate(Co-culture)?

20 May 2024 8,214 2 View

How about the efficiency of immunization with mRNA on mice or rabbits?

The development of mRNA vaccines on mice and rabbits?

18 May 2024 7,475 2 View

Why antibodies against EGFRvIII do not work?

We have constructed lentivirus transduced U251 cell lines to express (1) EGFRvIII (its amino acid sequence is MRPSGTAGAAFLALLAALCPASRALEEKKGNYVVTDHG..., which is identical to the sequence...

14 April 2024 2,370 1 View

How to get the intrinsic strength of cement gel?

11 March 2024 3,725 1 View

Have extreme weather events increased under the background of global warming?

It is a common belief that extreme weather and climate events increased in frequency and intensity over the last century or half a century, a time period that saw a rapid global climate warming....

16 February 2024 3,727 10 View

Does anyone know where I can find above-ground forest biomass data sets?

Does anyone know where I can find the data of measured above-ground biomass? I need the data of forest biomass measured on the spot for training and learning. If possible, I can also invest money...

12 November 2023 7,845 3 View

I have a question: How to apply a magnetic field in an electrolytic furnace?

How can we achieve the effect of the added magnetic field on the electrolysis process during molten salt electrolysis?

01 September 2023 4,416 2 View

Synthetic circadian clock or synthetic oscillators, suggest some great papers/groups?

I'd like to know what's current progress of 'synthetic circadian clock' or 'synthetic oscillators'. Indeed, I would like to investigate methods to regulate protein expression (e.g., transcritpion)...

24 August 2023 8,165 1 View

How will relocation of weather station affect data quality?

Weather stations are key facilities to record long-term change in surface air temperature (SAT) in land. However, some weather stations are located in or near cities, and they may suffer from...

20 June 2023 8,860 2 View

Absorption coefficient of methane?

Hello, Can anyone provide me with the absorption coefficient of methane gas at 7.7 um? Any reference?

06 August 2024 980 5 View

How can I add own Henry coefficients in Aspen Plus?

Hi, i would like to simulate an absorption process in Aspen Plus. I want to use the NRTL model und would like to add some individual Henry coefficients. Is that possible and how?

05 August 2024 2,333 2 View

Why is TLC not a form of partition chromatography?

Why is TLC not a form of partition chromatography and paper chromatography not a form of absorption chromatography?

03 August 2024 7,035 3 View

¿Are oxygen vacancies in semiconductors capable to change the band gap?

Dear all! Oxygen vacancies do raise negatively the Fermi level by increasing the negative charge in the semiconductor crystal structure. In the case of n-type semiconductors, I wonder if this...

30 July 2024 8,339 2 View

How to make the concentration measurements for the hydrogen absorption/desorption in the metal hydride?

Hello Everyone! I am trying to create a metal hydride system where I am trying to create a temperature control with the emphasis on measuring the concentration of the hydrogen with the hydrogen...

29 July 2024 7,577 2 View

Standard curve of H2O2?

Hi, I need to prepare a standard curve of H2O2 to evaluate the primary products of oxidation. H2O2 I have is 35%. How do I prepare a stock of H2O2 10 microlitere. If s.o has experience, I would be...

26 July 2024 3,460 2 View

What is single absorption ESR spectroscopy ?

In the context of Electron Spin Resonance (ESR) spectroscopy, under what conditions is an absorption spectrum considered to be a single absorption spectrum?

25 July 2024 2,325 1 View

The measurement of cellular uptake of nanoparticles?

What protocol can be used to measure the cellular uptake of a nanoparticle? Is there a way to get more of a non-targeted nanoparticle into the cell? Why a fluorescent nanoparticle does not enter...

18 July 2024 7,153 2 View

How to decide whether the refinement is correct or not, based on Rwp and Rexp factors by Fullprof?

One of the papers I read by Toby, where (title of the paper was "R factors in Rietveld analysis: How good is good enough?"), he tells us that to get good chi square value, you must have low Rwp,...

17 July 2024 9,668 4 View

What does an additional peak in FTIR between 3100 and 3300 nm affect the quality of the material, benzethonium chloride USP??

I do not know much about FTIR! The material Benzethonium Chloride USP was compared to a reference standard in regards to FTIR, as per the USP testing: Identification B. Infrared absorption (FTIR),...

16 July 2024 6,918 7 View