I aim to model an HPLC column by constructing a solvent reservoir within the Material Studio program to simulate real chromatographic conditions. Could you recommend a relevant tutorial or guidance on this topic?
We want to study senescence in adipocytes. However, we applied the method at adipose cells in our laboratory was as follows; ''Preadipocyte cell line 3T3-L1 (American Type Culture Collection,...
03 January 2024 2,653 1 View
Urgent question! Do human cells survive at -20 degrees for couple of weeks or do we have to be store them at -80 or below. Any suggestion will be greatly appreciated?
22 August 2023 1,864 5 View
We are experiencing a problem in the DW region despite successfully amplifying the UP region in OE-PCR. The Tm value of the forward primer is 63.4 (excluding the overlap region), and the Tm value...
10 May 2023 8,659 7 View
Hello everyone, I am trying to model a DCB (mode-1) test for a carbon-fiber composite sample on Ansys. However, I am very new to modelling and finite element analysis and I am a bit struggling....
06 October 2021 7,811 7 View
I need formulas of count of prime number and count of twin prime number . I know Gauss , Euler , Hardy - Littlewood work in this field . I need all study , formula , link whatever can be . please...
04 September 2021 1,607 4 View
Hello everyone, I am trying to model a DCB (mode-1) test for a carbon-fiber composite sample. However, I am a bit confused since I am very new to modelling and ABAQUS so I am looking for some...
02 September 2021 1,468 4 View
I am writing my thesis on the functionalization of a single-walled carbon nanotube with Fmoc-AA structures in NAMD software. My system consists of (30,30) chirality SWNT and 150 Fmoc-AA...
23 July 2021 1,580 2 View
We conducted a RCT of four arms, each corresponding to different versions of the internet-delivered 4-week (8 sessions, 2 per week) CBM intervention. Time Frame is baseline (pre-intervention),...
25 November 2020 6,700 3 View
I use modified Ellman's method for determine cholinesterase inhibitory activities of some compounds. But i suspect that some thiourea derivetives form thiol group and give false negative yellow...
05 March 2020 1,501 5 View
I plan to use ADNI brain MRI dataset whose data are in Nifti. SPM 12 has been used for segmentation (grey matter-white matter). I can visualize them but could not understand how to use them in...
10 February 2019 2,181 4 View
A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...
11 August 2024 9,801 2 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
Fatal error in MPI_Allreduce: Other MPI error, error stack: MPI_Allreduce(1628)......: MPI_Allreduce(sbuf=000002459254A180, rbuf=000002459F86A140, count=4851, MPI_DOUBLE_COMPLEX, MPI_SUM,...
09 August 2024 7,615 1 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
I need to do some experiment with it in liquid form.
07 August 2024 613 3 View
Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.
07 August 2024 8,782 3 View
What is the prepared reference material that can be used in the ICPE-9820 Shimadzu Japan instrument, which employs inductively coupled plasma optical emission spectrometry (ICP-OES) to measure...
06 August 2024 1,896 1 View
Are there any suggestions or insights you can provide to help address this problem
06 August 2024 1,443 2 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View