Dear Paulami,

You may conduct hydrolysis in the presence of sodium hydroxide (a base) or a strong acid such as sulfuric acid.

For more on these conditions, please see the publication contained in the following link:

http://www.mhhe.com/physsci/chemistry/carey/student/olc/graphics/carey04oc/ref/ch20reactionsamides.html

Or you may cleave the amide by amidase enzymes:

http://onlinelibrary.wiley.com/doi/10.1002/9783527639861.ch15/summary

Hoping this will be helpful,

Rafik

Dear Paulami,

You may conduct hydrolysis in the presence of sodium hydroxide (a base) or a strong acid such as sulfuric acid.

For more on these conditions, please see the publication contained in the following link:

http://www.mhhe.com/physsci/chemistry/carey/student/olc/graphics/carey04oc/ref/ch20reactionsamides.html

Or you may cleave the amide by amidase enzymes:

http://onlinelibrary.wiley.com/doi/10.1002/9783527639861.ch15/summary

Hoping this will be helpful,

Rafik

Dear Paulami,

As it known, amide is a very stable. So there is two ways :

1st : Hydrolysis  by strong acid or base in the presence of water.

2nd: This is slower and not a good one is by using water and excessive heat.

Best of luck 

Yazan 

Dear Paulami,

Breaking of amide bond can be achieved by using strong aq. base such as NaOH and or/ KOH (about 20% or more) and refluxing for some hours (5- 10h) or using strong acid like H2SO4 (70%) with heating at temp. (50-70 0C) for some hours (6 -8h).

A link of a paper on:  CATALYZED HYDROLYSIS OF AMIDE AND PEPTIDE BONDS IN PROTEINS is shown below:

http://nvlpubs.nist.gov/nistpubs/jres/29/jresv29n5p315_A1b.pdf

Best wishes

Please, find in attach, a paper on: THE KINETICS OF HYDROLYSIS OF THE AMIDE GROUP IN PROTEINS AND PEPTIDES PART 1. THE ACID HYDROLYSIS OF L-ASPARAGINE AND L-ASPARAGINYLGLYCINE

 Please, find in attach, an article on:Acidic and Basic Amide Hydrolysis

A link of a paper on:Recent perspectives concerning the mechanism of H3O+- and hydroxide-promoted amide hydrolysis is shown below:

http://pubs.acs.org/doi/abs/10.1021/ar00023a001

A link of a paper on: Hydrolysis of ester- and amide-type drugs by the purified isoenzymes of nonspecific carboxylesterase from rat liver is shown below:

http://www.sciencedirect.com/science/article/pii/000629528490176X

how to selectively cleave peptide bond? thanks.

Talaat I. El-Emary

I would be thankful to you if you provide me the reference for the following process:

"Breaking of amide bond can be achieved by using strong aq. base such as NaOH and or/ KOH (about 20% or more) and refluxing for some hours (5- 10h)"

I could not find this things in the article you provided. Thank you