We are conducting a large-scale inhibitor-based screen in transfected BaF3 cells, where we seed the cells in 384-well plates in the presence of different kinase inhibitors. The issue we are having is that all the cells often die after the seeding into 384 plates in a new media (also control cells). It happens often, but not every time and we have ruled out the problems related to media and serum change, seeding density (we seed 2500 cells in 50ul), mycoplasma contamination.

Has anyone experienced something similar?