Try to make cytospin preparations on slides.

Thanks Sabine Strehl. I need to grow them for a while so I guess cytospin is not the way forward - do you agree or my thinking is flawed? N

Hi Nitin,

So if I understand correctly you want to sort cells, culture them for a while and then stain them. I am not too familiar with the specific cells you described but maybe plating the sorted cells in chamber slides might be an option ?  

http://www.sigmaaldrich.com/catalog/product/sigma/s6815?lang=en®ion=GB

I think these slides are coated so your cells should adhere, when you are done you can fix them, take of the wells and do immuno. If attachment remains an issue you can try extra coating with for example Poly-Lysine/Fibronectin/ Gelatin or Collagen.

Good luck !

If you want to grow the cells cytospins are indeed no option. As pointed out by Pieter Louwe, growing the cells in chamber slides is worth trying.

Thanks Sabine Strehl and Pieter Louwe. 

These things have not worked for me. I must say I still need to give it a go with gelatin and collagen which I will do soon.