Pieter Louwe

5 Questions 20 Answers 0 Followers

Questions related from Pieter Louwe

PKH26-PCL phagocyte labelling dye mechanism?

Hi Everyone, PKH26-PCL dye has been used to label macrophages/phagocytes (http://www.sigmaaldrich.com/catalog/product/sigma/pkh26pcl?lang=en®ion=GB) I have been thinking about using this...

11 November 2017 5,386 1 View

Sudan black in cleared tisue?

I am using Rapiclear to clear the tissue after staining. However, there is still quite a bit of background an i was wondering if it would be possible to reduce this with Sudan black treatment...

11 November 2016 4,069 1 View

Is it possible to make vibratome sections of OCT embedded tissue?

I have some mouse heart tissue embedded in OCT. Usually we take cryosections but we have decided to try and make thicker sections with a vibratome. I was wondering if anyone has experience making...

10 October 2016 2,132 3 View

Cell death marker

Hi,Is anyone familiar with a stain for death cells in living zebrafish.More specifically I am looking for is a staining similar to Propidium Iodide (as in usable in live cells) but with a 405/647...

01 January 2016 9,081 1 View

Anyone familiar with an antibody to stain microglia in zebrafish?

I was wondering if anyone knows an antibody that can be used for staining microglia in zebrafish? I am familiar with 4c4 and L-plastin antibodies, but these are not perfect for me. Is anyone...

12 December 2015 988 9 View