Hello, I am new to computational chemistry. I am working on a project that requires that I attach ctma molecules to the phosphate groups of DNA molecule in AMBER MD. Is the a way I can do this using AMBER MD?
Despite not having cells in the media, I am getting purple color. I have tried many troubleshooting methods, varying media types, and even different MTTs from different companies to figure out the...
21 July 2024 9,914 1 View
I'm working with a hydrogel sample and I'd like to perform XRD analysis. Can anyone offer guidance on preparing the hydrogel for the XRD instrument? Specifically, I'm unsure about the best method...
20 July 2024 3,611 4 View
To help enchancing patients flow and service efficiency
18 July 2024 482 2 View
There exists a neural network model designed to predict a specific output, detailed in a published article. The model comprises 14 inputs, each normalized with minimum and maximum parameters...
14 July 2024 2,714 3 View
We are using MEGAshortscript™ T7 Transcription Kit for transcribing gRNAs from linearized plasmid templates. (MSP3485 digested with HindIII) (https://www.addgene.org/140082/) We let the reaction...
01 July 2024 6,342 0 View
Hello ResearchGate Community, How can Abaqus CAE be utilized to assess the variation in wall thickness throughout a plastic bottle following a blow molding simulation?
26 June 2024 9,281 4 View
MY RESEARCH TOPIC IS THE IMPACTS OF KIRI DAM ON SWEET POTATO CULTIVATION FOR FARMERS OF THE UPSTREAM COMMUNITIES, ADAMAWA STATE, NIGERIA. I NEED RELATED AND LATEST ARTICLES (2020 TO DATE) TO...
22 June 2024 4,117 1 View
Why the IF of almost all journals declined so much? For the file download go to https://www.researchgate.net/post/JCR_Impact_Factor_2024-Factors_causes_decline_in_IF
21 June 2024 3,358 0 View
Simulating a Hybrid System with PV, Grid, Electrolyzer (No Load) in HOMER. here is a question about how to use HOMER Pro to design a solar PV system for water electrolysis using electrolyzer for...
05 June 2024 5,609 0 View
It is possible for people specialized in music psychology to evaluate this questionnaire Through the link below Thank you https://forms.gle/qzoD7jN2UDUhMjwQ7
02 June 2024 9,546 0 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...
08 August 2024 8,329 0 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
Dear all, I am working on particle deposition in human's & rat's respiratory airways using CFD and I am looking for the 3D CAD file for my simulations (STEP or IGES format). If somone has such...
29 July 2024 1,092 2 View
I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.
29 July 2024 8,560 0 View
It has been documented that apoptotic cells themselves can induce phosphorylation of serine 139 on H2AX (γH2AX) due to DNA fragmentation during apoptosis (doi: 10.1074/jbc.275.13.9390). As γH2AX...
28 July 2024 7,983 2 View