Hi, you can find some examples with honeybees here

Toth, A. L., Kantarovich, S., Meisel, A. F., & Robinson, G. E. (2005). Nutritional status influences socially regulated foraging ontogeny in honey bees. Journal of Experimental Biology, 208(24), 4641-4649.

and references therein.

This protocol has been adapted also to Polistes paper wasps, such as in:

Toth, A. L., Bilof, K. B. J., Henshaw, M. T., Hunt, J. H., & Robinson, G. E. (2009). Lipid stores, ovary development, and brain gene expression in Polistes metricus females. Insectes Sociaux, 56(1), 77-84.

Bye bye,

AleCini

Thank you for this answer !

Dear colleague,

In insects, lipids were usually extracted following the procedure of Shibko et al. (1966)  and quantified by the sulfophosphovanillic method of Goldsworthy et al. (1972).

S. Shibko, P,Koivistoinen,C. Tratynec,A.Newhall, and L. Friedman, 1966. A method for the sequential quantitative separation and determination of protein, RNA, DNA, lipid and glycogen from a single rat liver homogenate or from subcellular fraction, Anal. Biochem. 19, 415 .

G. J. Goldsworthy, W. Mordue, and J. Guthkelch, Studies on insect adipokinetic hormones, Gen. Comp. Endocrinol. 18(3), 545 (1972).

 See details in some attached publications dealing with quantitative and qualitative analysis of lipids in several organs.

Best regards N. Soltani