Does anyone know of a cell fractionation and mitochondria extraction buffer with a simple composition, without deoxycholate?

More Juliette Vergnaud-Gauduchon's questions See All

Are there Web based tools to analyse 5'UTR variants?

I'm still unexperienced in this field and on ResearchGate. I need to analyze some variants that I found in the 5' upstream region. I would like to know if they occur in a pattern that could be a...

22 February 2024 932 0 View

What woud be the best clustering method?

Hello, Clustering methods are new to me: I'm trying to find out which would be the best clustering method for a sample size of around 350 observations and around fifteen variables, either...

01 July 2023 802 3 View

What sample size for Hierarchial Clustering Analysis?

What is the ideal sample size for an HCA? I read that this clustering method is best suited for small sample sizes, but would it be appropriate for a sample size of n=340 observations?

01 July 2023 7,558 4 View

Scale of impact of marine activities : desalinization and geothermal extraction?

I have to take decisions about some activities in marine protected areas - what level protection are we providing if these activities are occurring? I am looking for any good references on the...

06 November 2022 7,865 2 View

How to speed up spatial operations with R 'sf' package?

Hi everyone, I have to identify overlapping polygons, with one of the datasets containing thousands of polygons. I am using the sf package and its st_intersects function, as: dataframe1 %>%...

25 September 2022 9,633 1 View

Geometric standard error of the mean ?

Hi everybody, Graphpad prism is able to calculate geometric means and geometric standard deviation. - Is there any requirement for the data to have a normal distribution in order to calculate the...

14 July 2021 582 8 View

How can I fix residual covariances in MCMCglmm?

Hi, I need a little bit of help! I am working on a multivariate MCMC model, I basically want to model the relationship between a trait and fitness, with fitness modelled with a hurdle model. The...

28 February 2021 3,576 0 View

Has anyone used ITEX GC MS for benzaldehyde and 3 methyl butanal quantitation ?

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05 November 2019 5,568 5 View

What is the insertion site of the nosGal4-VP16 driver of the 3rd chromosome in drosophila (van doren, 1998)?

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08 September 2019 7,307 0 View

Which cell line would you recommend for a study of joint inflammation?

Hello everyone, I would like to make a short study on joint inflammation (especially MMP/TIMP production), which cell line would you recommend (and would be relatively easy to grow)? I have been...

08 February 2018 1,933 2 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

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Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

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RNA Extraction Using Hot Borate Method No Longer Working?

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Is there any way to quantify bacterial and fungal cells in their mixed culture?

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07 August 2024 7,535 4 View

Is changing the medium during the experiment overestimate the results?

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How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

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Why activated CAR-Jurkat cell could not kill targets?

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06 August 2024 641 2 View

The aqueous fraction of the hydroethanolic extract is showing the presence of palmitic acid. What is the mechanism responsible ?

Palmitic acid presence in aqueous fraction

05 August 2024 8,624 4 View

How can major oxides determined by ICP-MS/ES be normalized to 100% volatile free? How does this connect with LOI?

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Simon Lagies

Dear Juliette,

may I suggest you the following two articles:

for LC-MS:

Article Heat Stress-Induced Metabolic Remodeling in Saccharomyces cerevisiae

Or if you also have a GC avilable:

Article Metabolic profiling of isolated mitochondria and cytoplasm r...

I do not know of course if these protocols are suitable for your drug.

Best regards,

Simon

Ladan Ajdanian

Dear

This paper may help you

Article Sequential fractionation and isolation of subcellular protei...

Juliette Vergnaud-Gauduchon

Thank you all for your suggestions!