I have 5 treatment groups male mice sample and 5 treatment groups female mice, and I wanna to do western blotting to see if there any sex-dependent difference among markers (I need to compare male and female).

When I load the samples, do I have to load male and female in the same gel? I only have 20 wells in one gel, if I choose 2 samples for each groups, I do not have space to load markers. Can I just choose one samples from each group? or Can I load female and male in separate gel?

Thank you!