Statistical methods for comparing gene expression curves?

Hi all, I have generated two gene expression curves by measuring bioluminescence every 10 mins for 12 hrs. These curves tend to overlap at at some time points, while a difference can be seen at...

02 February 2021 2,613 7 View

In which solvent dissolve silver nanoparticles for XRD pattern study through NMR ?

The silver nanoparticles, which are solid form and I want to study the XRD pattern of this nanoparticles by Nuclear Magnetic Resonance (NMR), so I asked which solvent are used for dissolve of...

13 January 2021 3,434 4 View

Buckling of Smart composite plates using FEM ( MATLAB code)?

Can anyone help me with the FEM coding for Buckling of composite plates using the FSDT or HSDT - Source or the helping material (textbook, research paper etc) - Geometric stiffness matrix...

03 January 2021 3,059 3 View

How does one calculate interfacial heat transfer by using data from Fluent simulation for a subcooled flow boiling model?

I am trying to simulate subcooled boiling flow through an annulus using Fluent. Is it possible to get the values of interfacial heat transfer coefficients from either FLUENT report or CFD post?...

31 October 2020 3,599 3 View

Can someone help me with different watershed management practices for hilly areas ? Requirement is to have enough water for crop cultivation?

Can someone help me with different watershed management practices for hilly areas ? Requirement is to have enough water for crop cultivation. I am looking at the hilly areas, any proof of concept...

05 October 2020 5,641 15 View

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Due to COVID- 19, pupil teacher of teacher training programs are doing school internship in online mode which they are completely opposed to. What do you think as to it will adversely affect their...

09 August 2020 756 20 View

How best to get Cloud service credits from Cloud providers for my research simulations?

I need to run simulations running 100s of nodes in a simulated network and have to do that many times over. I could use Google Cloud Platform's $1000 provided for researchers doing PhD but I...

07 August 2020 4,537 3 View

Why is that only vaccine is available for Hepatitis B and not a drug that can eradicate it?

Hi Everyone, Entecavir 0.5 mg is usually given for patients with chronic hepatitis B to keep the virus under control and as well Vaccine is available for restricting the virus. If Entecavir 0.5...

22 June 2020 5,661 3 View

RT-PCR or Immunoassay? Or a combination?

So far, RT-PCR testing has been the frontline response to the COVID-19. PCR testing is considered as the gold standard for detecting COVID-19 and was designed by WHO, soon after the virus was...

21 April 2020 1,139 16 View

Is a combo of chloroquine and Zinc a cure for coronavirus?

Recent studies from Oklahoma Medical Research Foundation, University of Queensland Centre for Clinical Research, and Qingdao Municipal Hospital identified that Chloroquine phosphate, a drug used...

17 March 2020 6,929 94 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

Has anyone tried to scan fluorescence IHC slides with microarray scanner?

I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...

24 February 2021 9,210 1 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

No title

22 February 2021 9,932 11 View