I have tried to hydrolyze the sample using 2 M TFA at 100C for 24, 48 h and 120C for 2 h. From these conditions, the HPLC results showed that peaks were not separated into monosaccharide. I found that many papers using 2 M TFA for this analysis. I also have tried using 1 M H2SO4 at 100C, but it was failed too. Please, any suggestions, what other methods should I use for this analysis? Many thanks.
Mrs/Miss Muhardina,
Your experimental design should include mass spectrometric (MS) determination; however, within the framework of our own-authored (to me and my co-author's according to the authorship shown in the corresponding publications) innovative stochastic dynamic theory and model formulas for quantification of experimental MS variables.
Please, consider the content of the following discussion and the reference section shown, therein.
[https://www.researchgate.net/post/How_to_extract_and_purify_disaccharide_peptides_and_lactoyl_peptides_from_Mycobacteria_cell_wall]
Please, bear in mind, as well as, that the discussion focuses on MALDI-MS analysis; however, our stochastic dynamic method is applicable to different mass spectrometric ionization methods.
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