I've come across a protocol which mentions the ability to reduce protein (bovine serum albumin) dissolution rate in aqueous media through some "special treatment". Unfortunately, no mention is made as to what this special treatment consists of. Do any protocols exist for the modulation of dissolution rates of proteins such as BSA?
Ref in question is:
Hoth, Matthias, and Hans P. Merkle. "Formulation of silicone matrix systems for long term constant release of peptides." Drug development and industrial pharmacy 17.7 (1991): 985-999.
It depends on protein's characteristics and net charges! You could use slight acidic condition to increase the dissolution, and keep changing it in controlled manner to a window of small pH differences, better if it's done through a buffered mixture.
Actually, BSA stabilized the enzyme by hydrophobic interactions with the heated enzyme and that surface hydrophobicity is a major determinant of the extent of stabilization by a protein.
For a 10% (100 mg/mL) stock solution of BSA, dissolve 1 g powdered Fraction V or molecular biology grade BSA in 10 mL of distilled H2O; to avoid clumping, dissolve by layering the powder on the surface of the liquid. Gently rock the capped tube until the BSA has dissolved completely. Do not stir.
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