I would like to add a triglyceride solution and phosphatidylcholine to adherent cancer cells in serum-free media in order to study the short-term effects (~48 hrs). Can anyone suggest the best vehicle and/or protocol for doing so?
Can this pH of 8 be due to the presence of other substances? Or do I have an error somewhere in the analysis? Thank you!
26 June 2024 9,093 4 View
BD Pharmingen™ FITC Mouse Anti-Rat αβ T-Cell Receptor (561672) BD Pharmingen™ APC Mouse Anti-Rat CD4 (550057) BD Horizon™ V450 Rat anti-Mouse CD8a (560469) BD Pharmingen™ PE Mouse Anti-Rat CD45R...
10 May 2024 3,072 0 View
Dear colleagues, I am looking for advice on the validation of a standard questionnaire that I intend to translate. The original version contains 40 items. Could you please tell me what sample...
26 March 2024 1,660 11 View
So I have ben trying to prepare TiO2 nanoparticles but i am getting trouble with the fromation of White PPT which later dissolves to form a clear solution that unfortunately doesnt form a gel. it...
25 March 2024 8,808 2 View
For both heat-induced or proteolytic-induced antigen retrieval, does the AR needs to be done on the same day of the IF? Or can be done one day in advance, store the slides at 4º, and performe the...
06 March 2024 220 1 View
Any empirical study about theater education for immigrant students?
03 March 2024 6,134 0 View
Currently, I am trying to model packers made from recycled plastic material (E=360N/mm^2 and a poisson ratio of 0.4). The best way to model these packers would be to only adjust the contact...
26 February 2024 5,699 1 View
Optimal buffer and conditions to determine the enzymatic activity of MPO by the colorimetric method that is based on the formation of hypochlrous acid which reacts with taurine to form the...
07 February 2024 3,056 0 View
In Guassian, which convergence algorithm is best for working with transition metal interaction? It is taking a long time to even get the first block of coordinates, in the output file it said to...
28 January 2024 4,276 1 View
I need to evaluate abdominal adipose tissue hypertrophy in a murine model with metabolic syndrome, with and without treatment and I need to review which is the best objective to measure adipocyte...
09 January 2024 7,149 0 View
"PUBLISHING IN A SCOPUS JOURNAL" Researchers are now at a cross road. The critical need to publish in a Scopus or ISI, etc journal is ever vital. Journal Publication fees must be submitted....
10 August 2024 8,621 1 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have an antibody binding generic protein and I need to compare its activity in a free and immobolized form. I understand that there are a number of methods to determine Kd value of a free...
05 August 2024 5,311 0 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View