I would like to add a triglyceride solution and phosphatidylcholine to adherent cancer cells in serum-free media in order to study the short-term effects (~48 hrs). Can anyone suggest the best vehicle and/or protocol for doing so?
I asked recommended real-time or nearly real-time data source for any parameters for flood risk on a microscale analysis for urban area cases in developing countries that had agglomeration urban...
18 February 2021 2,617 2 View
Someone knows about a specific law or regulation which mentions the use of the rose bengal chloramphenicol agar is suitable to isolate and count fungi. Perhaps, someone has done a research about...
07 January 2021 5,325 5 View
07 January 2021 9,052 1 View
I have a repeated measures design with about 16 cases and 5-6 points of measuring. Sometimes, 1-4 full cases or some points of measure are missing. (The measures are 20 numerical and categorical...
20 December 2020 9,004 7 View
I am using MnFe2O4 NPs as adsorbent for CrVI for wastewater treatment following literatures as it is one of the best, but I have a challenge of releasing Mn+2 in the environment. A lot of...
19 November 2020 3,404 4 View
Hello, I have some troubles with the synthesis of a DNA probe for FISH in paraffin tissues, we decide to synthesize it by PCR with labelled dUTP, but we haven't had any results, I tried chaging...
08 September 2020 183 3 View
Hi, Does anyone have experience with transfecting HAP1 cells for genome editing with Cas9/gRNA complexes? Or are the HAP1 cells difficult to transfect? Thanks.
27 August 2020 6,028 1 View
After being affected with that viruse , most of the patients feel depressed and sad . How can we , psychologically, encourage them ?
26 August 2020 6,178 93 View
Dear researchers, The COVID-19 pandemic has large societal and economic impacts, from which also different stakeholders involved in EU H2020 energy projects are affected. With this survey, we...
17 June 2020 4,542 4 View
Has anyone ever received the error 'Illegal storage access Abnormal completion' from MIKE? I am using the Data Extractor FM and at certain times this error comes up. Once I receive this error I...
24 March 2020 2,518 3 View
Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...
03 March 2021 5,999 3 View
We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...
02 March 2021 6,036 1 View
02 March 2021 3,060 3 View
If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis
02 March 2021 7,670 3 View
I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...
02 March 2021 9,945 3 View
im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...
02 March 2021 2,246 2 View
during ultrasound examination of acute abdomen, sometimes we see free fluid , can we know the type of fluid ,whether blood or not , using ultrasound only? if yes , what are the sonographic...
01 March 2021 7,476 4 View
Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...
01 March 2021 3,867 3 View
Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?
01 March 2021 8,087 3 View
We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...
01 March 2021 3,355 3 View