I want to add sodium bicarbonate in RPMi.
I have sodium bicarbonate concentrate and it says 0.1M eluent concentrate for IC.
Can I use it at human cell culture?
How can I add sodium bicarbonate in RPMI?
I heard that by adding it, it will change pH..
adjust pH, autoclave, and add it to RPMI is right?
Please give me a help.
Thank you!
The recommended method is to add the bicarb to the RPMI medium, adjust the pH, then filtration sypterilize the whole solution. Alternatively, if the RPMI is already dissolved and sterile, add sterile bicarb solution and use sterile acid solution to adjust the pH. To test the pH, measure pH using a small aliquot or use a sterile pipette and deposit droplets onto pH paper.
Ellen A G Chernoff Thanks for answer! Is there pH change after autoclave biocarb solution? Or just filtering is enough?