How to add single dTTP to double strand DNA?

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Adam B Shapiro

Can you use dideoxyTTP? That would prevent further nucleotide addition.

Guobing Xiang

Terminal transferase (TdT) is a template independent polymerase that catalyzes the addition of deoxynucleotides to the 3' hydroxyl terminus of DNA molecules. Protruding, recessed or blunt-ended double or single-stranded DNA molecules serve as a substrate for TdT.

Terminal Transferase | NEB

📷www.neb.com/products/m0315-terminal-transferase